Hepatitis B Surface Antigen Quantity Positively Correlates with Plasma Levels of microRNAs Differentially Expressed in Immunological Phases of Chronic Hepatitis B in Children

Thilde Nordmann Winther, Ida Louise Heiberg, Claus Heiner Bang-Berthelsen, Flemming Pociot, Birthe Hogh, Jason Blackard (Editor)

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Background and Aim: Children with chronic hepatitis B (CHB) are at high risk of progressive liver disease. It is suggested that a newly-identified panel of 16 microRNAs is important in the pathogenesis of CHB in children. Subviral hepatitis B surface antigen (HBsAg) particles are produced in large excess over infectious virions. Interestingly, circulating HBsAg particles have been shown to carry microRNAs. A thorough characterisation of the identified microRNAs and HBsAg over time in plasma from children with CHB may provide useful information about the natural course of childhood CHB.Patients and Methods: A cohort of 42 children with CHB was followed over time. Three to five blood samples were obtained from each child at minimum intervals of half a year; in total 180 blood samples. Plasma levels of the 16 microRNAs previously identified were analysed by quantitative real-time polymerase-chain-reaction. Plasma HBsAg was quantified using ARCHITECT (R) HBsAg assay.Results: The presence of 14/16 plasma microRNAs in children with CHB was confirmed. All 14 microRNAs were significantly differentially expressed in different immunological phases of the disease. MicroRNA plasma levels were highest in immune-tolerant children, lower in immune-active children, and reached the lowest values in immune-inactive children, p
Original languageEnglish
Article numbere80384
JournalP L o S One
Issue number11
Number of pages13
Publication statusPublished - 2013
Externally publishedYes


  • Agricultural and Biological Sciences (all)
  • Biochemistry, Genetics and Molecular Biology (all)
  • Medicine (all)
  • hepatitis B surface antigen
  • microRNA
  • microRNA 100 5p
  • microRNA 122 3p
  • microRNA 122 5p
  • microRNA 1247 5p
  • microRNA 125b 5p
  • microRNA 192 3p
  • microRNA 192 5p
  • microRNA 193b 3p
  • microRNA 194 5p
  • microRNA 215
  • microRNA 365a 3p
  • microRNA 455 3p
  • microRNA 455 5p
  • microRNA 483 3p
  • microRNA 855 5p
  • microRNA 99a 5p
  • unclassified drug
  • adolescent
  • age
  • article
  • blood sampling
  • child
  • chronic hepatitis
  • chronic hepatitis B
  • clinical article
  • cohort analysis
  • controlled study
  • female
  • hepatitis B
  • human
  • immunoassay
  • immunological tolerance
  • immunoreactivity
  • longitudinal study
  • male
  • protein blood level
  • quantitative analysis
  • real time polymerase chain reaction
  • blood
  • immunology
  • infant
  • preschool child
  • Adolescent
  • Child
  • Child, Preschool
  • Female
  • Hepatitis B Surface Antigens
  • Hepatitis B, Chronic
  • Humans
  • Infant
  • Male
  • MicroRNAs
  • Genetics - General
  • Genetics - Human
  • Pathology - General
  • Digestive system - Physiology and biochemistry
  • Digestive system - Pathology
  • Blood - Blood and lymph studies
  • Blood - Blood cell studies
  • Pediatrics
  • Genetics of bacteria and viruses
  • Virology - General and methods
  • Immunology - General and methods
  • Medical and clinical microbiology - Virology
  • DNA and RNA Reverse Transcribing Viruses, Microorganisms, Viruses
  • Animals, Chordates, Humans, Mammals, Primates, Vertebrates
  • immunological phases

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