Hepatic UDP-glucose 13C isotopomers from [U-13C]glucose: A simple analysis by 13C NMR of urinary menthol glucuronide

Ana Carina Loureiro Mendes, M. Madalena Caldeira, Claudia Silva, Shawn C. Burgess, Matthew E. Merritt, Filipe Gomes, Cristina Barosa, Teresa C. Delgado, Fatima Franco, Pedro Monteiro, Luis Providencia, John G. Jones

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Menthol glucuronlide was isolated from the urine of a healthy-70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-C-13]glucose. Glucuronide C-13-excess enrichment levels were 4-6% and thus provided high signa to-noise ratios (SNRs) for confident assignment of C-13-C-13 Spin-coupled multiplet components within each C-13 resonance by C-13 NMR. The [U-C-13]glucuronide isotopomer derived via dire pathway conversion of [U-C-13]glucose to [U-C-13] UDP-glucose was resolved from [1,2,3-C-13(3)]- and [1,2-C-13(2)]glucuronide is topomers derived via Cori cycle or indirect pathway metabolism of [U-C-13]glucose. In a second study, a group of four overnight-fasted patients (63 +/- 10 kg) with severe heart failure were give peppermint oil and infused with [U-C-13]glucose for 4 hr (14 mg/kg prime, 0.12 mg/kg/min constant infusion) resulting in a steady-state plasma [U-C-13]glucose enrichment of 4.6% +/- 0.6%. Menthol glucuronide was harvested and glucuronide C-13-isotopomers were analyzed by C-13 NMR. [U-C-13]glucuronide enrichment was 0.6% +/- 0.1%, and the sum of [1,2,3-C-13(3)] an [1,2-C-13(2)]glucuronide enrichments was 0.9% +/- 0.2%. Fro these data, flux of plasma glucose to hepatic UDPG was est mated to be 15% +/- 4% that of endogenous glucose production (EGP), and the Cori cycle accounted for at least 32% +/- 10% of GP.
Original languageEnglish
JournalMagnetic Resonance in Medicine
Volume56
Issue number5
Pages (from-to)1121-1125
Number of pages5
ISSN0740-3194
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • carbon 13
  • glucose
  • glucuronide
  • glycogen
  • isotope
  • isotopomer
  • menthol glucuronide
  • peppermint oil
  • unclassified drug
  • uridine diphosphate glucose
  • adult
  • article
  • carbon nuclear magnetic resonance
  • citric acid cycle
  • cori cycle
  • diet restriction
  • female
  • gluconeogenesis
  • glucose blood level
  • glucose metabolism
  • glucose transport
  • glycogen metabolism
  • heart failure
  • human
  • human experiment
  • liver metabolism
  • normal human
  • signal noise ratio
  • steady state
  • urine level
  • Adult
  • Blood Glucose
  • Carbon Isotopes
  • Female
  • Gluconeogenesis
  • Glucose
  • Glucuronates
  • Humans
  • Liver
  • Magnetic Resonance Spectroscopy
  • Menthol
  • Uridine Diphosphate Glucose
  • 13C-isotopomer
  • Cori cycle
  • Glycogen
  • Indirect pathway
  • Krebs cycle
  • RADIOLOGY,
  • GLUCONEOGENIC FLUXES
  • GLYCOGEN TURNOVER
  • CORI CYCLE
  • METABOLISM
  • HUMANS
  • LIVER
  • SPECTROSCOPY
  • GLUTAMATE
  • H-2
  • ACETAMINOPHEN
  • C-13-isotopomer
  • indirect pathway
  • Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common female
  • glucose 58367-01-4 metabolism
  • glucuronide isotopomer
  • 10068, Biochemistry studies - Carbohydrates
  • 12504, Pathology - Diagnostic
  • 14004, Digestive system - Physiology and biochemistry
  • 14006, Digestive system - Pathology
  • 14506, Cardiovascular system - Heart pathology
  • 15504, Urinary system - Physiology and biochemistry
  • Cardiovascular Medicine
  • Gastroenterology
  • Methods and Techniques
  • heart failure Heart Failure, Congestive (MeSH) heart disease diagnosis
  • signal-to-noise ratio
  • Human Medicine, Medical Sciences
  • liver digestive system
  • urine excretory system
  • NMR laboratory techniques, spectrum analysis techniques

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