A huge shortage of liver organs for transplantation has motivated the research field of tissue engineering to develop bioartificial liver tissue and even a whole liver. The goal of NanoBio4Trans is to create a vascularized bioartificial liver tissue, initially as a liver-support system. Due to limitations of primary hepatocytes regarding availability and maintenance of functionality, stem cells and especially human induced pluripotent stem cells (hIPS cells) are an attractive cell source for liver tissue engineering. The aim of this part of NanoBio4Trans is to optimize culture and hepatic differentiation of hIPS-derived definitive endoderm (DE) cells in a 3D porous polymer scaffold built-in a perfusable bioreactor. The use of a microfluidic bioreactor array enables the culture of 16 independent tissues in one experimental run and thereby an optimization study to be performed.
|Number of pages||1|
|Publication status||Published - 2014|
|Event||3rd International Conference on Tissue Science & Regenerative Medicine - Valencia Convention Center, Valencia, Spain|
Duration: 24 Sep 2014 → 26 Sep 2014
|Conference||3rd International Conference on Tissue Science & Regenerative Medicine|
|Location||Valencia Convention Center|
|Period||24/09/2014 → 26/09/2014|
Hemmingsen, M., Muhammad, H. B., Mohanty, S., Wolff, A., Emnéus, J., Aspegren, A., & Dufva, M. (2014). Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused 3D Porous Polymer Scaffold for Liver Tissue Engineering. Poster session presented at 3rd International Conference on Tissue Science & Regenerative Medicine, Valencia, Spain.