Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused 3D Porous Polymer Scaffold for Liver Tissue Engineering

Mette Hemmingsen, Haseena Bashir Muhammad, Soumyaranjan Mohanty, Anders Wolff, Jenny Emnéus, Anders Aspegren, Martin Dufva

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    Abstract

    A huge shortage of liver organs for transplantation has motivated the research field of tissue engineering to develop bioartificial liver tissue and even a whole liver. The goal of NanoBio4Trans is to create a vascularized bioartificial liver tissue, initially as a liver-support system. Due to limitations of primary hepatocytes regarding availability and maintenance of functionality, stem cells and especially human induced pluripotent stem cells (hIPS cells) are an attractive cell source for liver tissue engineering. The aim of this part of NanoBio4Trans is to optimize culture and hepatic differentiation of hIPS-derived definitive endoderm (DE) cells in a 3D porous polymer scaffold built-in a perfusable bioreactor. The use of a microfluidic bioreactor array enables the culture of 16 independent tissues in one experimental run and thereby an optimization study to be performed.
    Original languageEnglish
    Publication date2014
    Number of pages1
    Publication statusPublished - 2014
    Event3rd International Conference on Tissue Science & Regenerative Medicine - Valencia Convention Center, Valencia, Spain
    Duration: 24 Sep 201426 Sep 2014

    Conference

    Conference3rd International Conference on Tissue Science & Regenerative Medicine
    LocationValencia Convention Center
    CountrySpain
    CityValencia
    Period24/09/201426/09/2014

    Cite this

    Hemmingsen, M., Muhammad, H. B., Mohanty, S., Wolff, A., Emnéus, J., Aspegren, A., & Dufva, M. (2014). Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused 3D Porous Polymer Scaffold for Liver Tissue Engineering. Poster session presented at 3rd International Conference on Tissue Science & Regenerative Medicine, Valencia, Spain.