Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis

L.M. Blank; Brian Jensen Købmann; Ole Michelsen; L.K. Nielsen; Peter Ruhdal Jensen

Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis

L.M. Blank, Brian Jensen Købmann, Ole Michelsen, L.K. Nielsen, Peter Ruhdal Jensen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.

Original language	English
Journal	Journal of Bacteriology
Volume	183
Issue number	22
Pages (from-to)	6707-6709
ISSN	0021-9193
Publication status	Published - 2001

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title = "Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis",

abstract = "H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.",

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author = "L.M. Blank and K{\o}bmann, {Brian Jensen} and Ole Michelsen and L.K. Nielsen and Jensen, {Peter Ruhdal}",

year = "2001",

language = "English",

volume = "183",

pages = "6707--6709",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis

AU - Blank, L.M.

AU - Købmann, Brian Jensen

AU - Michelsen, Ole

AU - Nielsen, L.K.

AU - Jensen, Peter Ruhdal

PY - 2001

Y1 - 2001

N2 - H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.

AB - H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.

KW - gram

M3 - Journal article

VL - 183

SP - 6707

EP - 6709

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 22

ER -