Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis

L.M. Blank, Brian Jensen Købmann, Ole Michelsen, L.K. Nielsen, Peter Ruhdal Jensen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.
    Original languageEnglish
    JournalJournal of Bacteriology
    Volume183
    Issue number22
    Pages (from-to)6707-6709
    ISSN0021-9193
    Publication statusPublished - 2001

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