Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates

Maria Angeles Argudin, Birgit Lauzat, Britta Kraushaar, Patricia Alba, Lina Cavaco, Patrick Butaye, M. Concepción Porrero, Antonio Battisti, Bernd-Alois Tenhagen, Alexandra Fetsch, Beatriz Guerra

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has emerged in animal production worldwide. Most LA-MRSA in Europe belong to the clonal complex (CC)398. The reason for the LA-MRSA emergence is not fully understood. Besides antimicrobial agents used for therapy, other substances with antimicrobial activity applied in animal feed, including metal-containing compounds might contribute to their selection. Some of these genes have been found in various novel SCCmec cassettes. The aim of this study was to assess the occurrence of metal-resistance genes among a LA-S. aureus collection [n = 554, including 542 MRSA and 12 methicillin-susceptible S. aureus (MSSA)] isolated from livestock and food thereof. Most LA-MRSA isolates (76%) carried at least one metal-resistance gene. Among the LA-MRSA CC398 isolates (n = 456), 4.8%, 0.2%, 24.3% and 71.5% were positive for arsA (arsenic compounds), cadD (cadmium), copB (copper) and czrC (zinc/cadmium) resistance genes, respectively. In contrast, among the LA-MRSA non-CC398 isolates (n = 86), 1.2%, 18.6% and 16.3% were positive for the cadD, copB and czrC genes, respectively, and none were positive for arsA. Of the LA-MRSA CC398 isolates, 72% carried one metal-resistance gene, and the remaining harboured two or more in different combinations. Differences between LA-MRSA CC398 and non-CC398 were statistically significant for arsA and czrC. The czrC gene was almost exclusively found (98%) in the presence of SCCmec V in both CC398 and non-CC398 LA-MRSA isolates from different sources. Regarding the LA-MSSA isolates (n = 12), some (n = 4) were also positive for metal-resistance genes. This study shows that genes potentially conferring metal-resistance are frequently present in LA-MRSA.
Original languageEnglish
JournalVeterinary Microbiology
Volume191
Pages (from-to)88–95
ISSN0378-1135
DOIs
Publication statusPublished - 2016

Keywords

  • Zinc
  • Copper
  • Arsenic compounds
  • Cadmium
  • SCCmec

Cite this

Argudin, Maria Angeles ; Lauzat, Birgit ; Kraushaar, Britta ; Alba, Patricia ; Cavaco, Lina ; Butaye, Patrick ; Porrero, M. Concepción ; Battisti, Antonio ; Tenhagen, Bernd-Alois ; Fetsch, Alexandra ; Guerra, Beatriz. / Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates. In: Veterinary Microbiology. 2016 ; Vol. 191. pp. 88–95.
@article{69f6de8e2e56495ebe612540e1f93644,
title = "Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates",
abstract = "Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has emerged in animal production worldwide. Most LA-MRSA in Europe belong to the clonal complex (CC)398. The reason for the LA-MRSA emergence is not fully understood. Besides antimicrobial agents used for therapy, other substances with antimicrobial activity applied in animal feed, including metal-containing compounds might contribute to their selection. Some of these genes have been found in various novel SCCmec cassettes. The aim of this study was to assess the occurrence of metal-resistance genes among a LA-S. aureus collection [n = 554, including 542 MRSA and 12 methicillin-susceptible S. aureus (MSSA)] isolated from livestock and food thereof. Most LA-MRSA isolates (76{\%}) carried at least one metal-resistance gene. Among the LA-MRSA CC398 isolates (n = 456), 4.8{\%}, 0.2{\%}, 24.3{\%} and 71.5{\%} were positive for arsA (arsenic compounds), cadD (cadmium), copB (copper) and czrC (zinc/cadmium) resistance genes, respectively. In contrast, among the LA-MRSA non-CC398 isolates (n = 86), 1.2{\%}, 18.6{\%} and 16.3{\%} were positive for the cadD, copB and czrC genes, respectively, and none were positive for arsA. Of the LA-MRSA CC398 isolates, 72{\%} carried one metal-resistance gene, and the remaining harboured two or more in different combinations. Differences between LA-MRSA CC398 and non-CC398 were statistically significant for arsA and czrC. The czrC gene was almost exclusively found (98{\%}) in the presence of SCCmec V in both CC398 and non-CC398 LA-MRSA isolates from different sources. Regarding the LA-MSSA isolates (n = 12), some (n = 4) were also positive for metal-resistance genes. This study shows that genes potentially conferring metal-resistance are frequently present in LA-MRSA.",
keywords = "Zinc, Copper, Arsenic compounds, Cadmium, SCCmec",
author = "Argudin, {Maria Angeles} and Birgit Lauzat and Britta Kraushaar and Patricia Alba and Lina Cavaco and Patrick Butaye and Porrero, {M. Concepci{\'o}n} and Antonio Battisti and Bernd-Alois Tenhagen and Alexandra Fetsch and Beatriz Guerra",
year = "2016",
doi = "10.1016/j.vetmic.2016.06.004",
language = "English",
volume = "191",
pages = "88–95",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",

}

Argudin, MA, Lauzat, B, Kraushaar, B, Alba, P, Cavaco, L, Butaye, P, Porrero, MC, Battisti, A, Tenhagen, B-A, Fetsch, A & Guerra, B 2016, 'Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates', Veterinary Microbiology, vol. 191, pp. 88–95. https://doi.org/10.1016/j.vetmic.2016.06.004

Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates. / Argudin, Maria Angeles; Lauzat, Birgit; Kraushaar, Britta; Alba, Patricia; Cavaco, Lina; Butaye, Patrick; Porrero, M. Concepción; Battisti, Antonio; Tenhagen, Bernd-Alois; Fetsch, Alexandra; Guerra, Beatriz.

In: Veterinary Microbiology, Vol. 191, 2016, p. 88–95.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates

AU - Argudin, Maria Angeles

AU - Lauzat, Birgit

AU - Kraushaar, Britta

AU - Alba, Patricia

AU - Cavaco, Lina

AU - Butaye, Patrick

AU - Porrero, M. Concepción

AU - Battisti, Antonio

AU - Tenhagen, Bernd-Alois

AU - Fetsch, Alexandra

AU - Guerra, Beatriz

PY - 2016

Y1 - 2016

N2 - Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has emerged in animal production worldwide. Most LA-MRSA in Europe belong to the clonal complex (CC)398. The reason for the LA-MRSA emergence is not fully understood. Besides antimicrobial agents used for therapy, other substances with antimicrobial activity applied in animal feed, including metal-containing compounds might contribute to their selection. Some of these genes have been found in various novel SCCmec cassettes. The aim of this study was to assess the occurrence of metal-resistance genes among a LA-S. aureus collection [n = 554, including 542 MRSA and 12 methicillin-susceptible S. aureus (MSSA)] isolated from livestock and food thereof. Most LA-MRSA isolates (76%) carried at least one metal-resistance gene. Among the LA-MRSA CC398 isolates (n = 456), 4.8%, 0.2%, 24.3% and 71.5% were positive for arsA (arsenic compounds), cadD (cadmium), copB (copper) and czrC (zinc/cadmium) resistance genes, respectively. In contrast, among the LA-MRSA non-CC398 isolates (n = 86), 1.2%, 18.6% and 16.3% were positive for the cadD, copB and czrC genes, respectively, and none were positive for arsA. Of the LA-MRSA CC398 isolates, 72% carried one metal-resistance gene, and the remaining harboured two or more in different combinations. Differences between LA-MRSA CC398 and non-CC398 were statistically significant for arsA and czrC. The czrC gene was almost exclusively found (98%) in the presence of SCCmec V in both CC398 and non-CC398 LA-MRSA isolates from different sources. Regarding the LA-MSSA isolates (n = 12), some (n = 4) were also positive for metal-resistance genes. This study shows that genes potentially conferring metal-resistance are frequently present in LA-MRSA.

AB - Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has emerged in animal production worldwide. Most LA-MRSA in Europe belong to the clonal complex (CC)398. The reason for the LA-MRSA emergence is not fully understood. Besides antimicrobial agents used for therapy, other substances with antimicrobial activity applied in animal feed, including metal-containing compounds might contribute to their selection. Some of these genes have been found in various novel SCCmec cassettes. The aim of this study was to assess the occurrence of metal-resistance genes among a LA-S. aureus collection [n = 554, including 542 MRSA and 12 methicillin-susceptible S. aureus (MSSA)] isolated from livestock and food thereof. Most LA-MRSA isolates (76%) carried at least one metal-resistance gene. Among the LA-MRSA CC398 isolates (n = 456), 4.8%, 0.2%, 24.3% and 71.5% were positive for arsA (arsenic compounds), cadD (cadmium), copB (copper) and czrC (zinc/cadmium) resistance genes, respectively. In contrast, among the LA-MRSA non-CC398 isolates (n = 86), 1.2%, 18.6% and 16.3% were positive for the cadD, copB and czrC genes, respectively, and none were positive for arsA. Of the LA-MRSA CC398 isolates, 72% carried one metal-resistance gene, and the remaining harboured two or more in different combinations. Differences between LA-MRSA CC398 and non-CC398 were statistically significant for arsA and czrC. The czrC gene was almost exclusively found (98%) in the presence of SCCmec V in both CC398 and non-CC398 LA-MRSA isolates from different sources. Regarding the LA-MSSA isolates (n = 12), some (n = 4) were also positive for metal-resistance genes. This study shows that genes potentially conferring metal-resistance are frequently present in LA-MRSA.

KW - Zinc

KW - Copper

KW - Arsenic compounds

KW - Cadmium

KW - SCCmec

U2 - 10.1016/j.vetmic.2016.06.004

DO - 10.1016/j.vetmic.2016.06.004

M3 - Journal article

VL - 191

SP - 88

EP - 95

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -