Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover

M. Manefield, Thomas Bovbjerg Rasmussen, M. Henzter, Jens Bo Andersen, P. Steinberg, S. Kjelleberg, Michael Christian Givskov

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    Abstract

    N-acyl-L-homoserine lactones (AHLs) are co-regulatory ligands required for control of the expression of genes encoding virulence traits in many Gram-negative bacterial species. Recent studies have indicated that AHLs modulate the cellular concentrations of LuxR-type regulatory proteins by binding and fortifying these proteins against proteolytic degradation (Zhu & Winans, 2001). Halogenated furanones produced by the macroallga Delisea pulchra inhibit AHL-dependent gene expression. This study assayed for an in vivo interaction between a tritiated halogenated furanone and the LuxR protein of Vibrio fischeri overproduced in Escherichia coli. Whilst a stable interaction between the algal metabolite and the bacterial protein was not found, it was noted by Western analysis that the half-life of the protein is reduced up to 100-fold in the presence of halogenated furanones. This suggests that halogenated furanones modulate LuxR activity but act to destabilize, rather than protect, the AHL-dependent transcriptional activator. The furanone-dependent reduction in the cellular concentration of the LuxR protein was associated with a reduction in expression of a plasmid encoded P-luxl-gfp(ASV) fusion suggesting that the reduction in LuxR concentration is the mechanism by which furanones control expression of AHL-dependent phenotypes. The mode of action by which halogenated furanones reduce cellular concentrations of the LuxR protein remains to be characterized.
    Original languageEnglish
    JournalMicrobiology-Sgm
    Volume148
    Pages (from-to)1119-1127
    ISSN1350-0872
    Publication statusPublished - 2002

    Cite this

    Manefield, M., Rasmussen, T. B., Henzter, M., Andersen, J. B., Steinberg, P., Kjelleberg, S., & Givskov, M. C. (2002). Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover. Microbiology-Sgm, 148, 1119-1127.