H2BC: a new technique for NMR analysis of complex carbohydrates

Bent O. Petersen, E. Vinogradov, W. Kay, P. Wurtz, NT Nyberg, Jens Øllgaard Duus, OW Sorensen

Research output: Contribution to journalJournal articleResearchpeer-review


It is demonstrated that the H2BC NMR pulse sequence (J Am. Chem. Soc. 2005, 127, 6154, Magn. Reson. Chem. 2005, 43, 971-974) offers unambiguous assignments and significant simplification of NMR spectra of large and complex carbohydrates compared to other techniques for the establishment of correlations over more than one bond. H2BC almost exclusively correlates protons and proton-bearing carbon spins separated by two covalent bonds and is independent of occasionally vanishing (2)J(CH) coupling constants, which alleviates the problem of missing two-bond correlations in HMBC spectra. H2BC also solves the problem of distinguishing two- and three-bond correlations in HSQC-TOCSY or HMBC. It is a further asset of H2BC that the experiment is significantly shorter than HMBC and HSQC-TOCSY, and hence less sensitive to transverse relaxation. The H2BC experiment is demonstrated on an approximately 30-residue oligosaccharide from Francisella victoria. (c) 2005 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalCarbohydrate Research
Issue number4
Pages (from-to)550-556
Number of pages7
Publication statusPublished - 2006
Externally publishedYes


  • Carbohydrates
  • Carbon Isotopes
  • Francisella
  • Hydrogen
  • Magnetic Resonance Spectroscopy
  • Oligosaccharides
  • Polysaccharides, Bacterial
  • 7YNJ3PO35Z Hydrogen
  • Bond strength (chemical)
  • Carbon
  • Correlation methods
  • Demonstrations
  • Nuclear magnetic resonance
  • Oligomers
  • Protons
  • carbohydrate derivative
  • carbon
  • oligosaccharide
  • proton
  • article
  • bacterium
  • complex formation
  • correlation analysis
  • covalent bond
  • Francisella victoria
  • heteronuclear 2 bond correlation nuclear magnetic resonance
  • nuclear magnetic resonance
  • priority journal
  • technique
  • Bacteria (microorganisms)
  • 13C
  • 1H
  • H2BC
  • Oligosaccharide
  • NMR
  • T
  • H-1
  • C-13
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Negative Aerobic Rods and Cocci [06500] Francisella victoria species
  • complex carbohydrates
  • oligosaccharides
  • 10060, Biochemistry studies - General
  • 10068, Biochemistry studies - Carbohydrates
  • 31000, Physiology and biochemistry of bacteria
  • heteronuclear 2-bond correlation-nuclear magnetic resonance H2BC-NMR laboratory techniques, spectrum analysis techniques
  • Biochemistry and Molecular Biophysics
  • Methods and Techniques


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