H2BC: a new technique for NMR analysis of complex carbohydrates

Bent O.  Petersen; E. Vinogradov; W. Kay; P. Wurtz; NT Nyberg; Jens Øllgaard Duus; OW Sorensen

doi:10.1016/j.carres.2005.11.020

H2BC: a new technique for NMR analysis of complex carbohydrates

Bent O. Petersen, E. Vinogradov, W. Kay, P. Wurtz, NT Nyberg, Jens Øllgaard Duus, OW Sorensen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

It is demonstrated that the H2BC NMR pulse sequence (J Am. Chem. Soc. 2005, 127, 6154, Magn. Reson. Chem. 2005, 43, 971-974) offers unambiguous assignments and significant simplification of NMR spectra of large and complex carbohydrates compared to other techniques for the establishment of correlations over more than one bond. H2BC almost exclusively correlates protons and proton-bearing carbon spins separated by two covalent bonds and is independent of occasionally vanishing (2)J(CH) coupling constants, which alleviates the problem of missing two-bond correlations in HMBC spectra. H2BC also solves the problem of distinguishing two- and three-bond correlations in HSQC-TOCSY or HMBC. It is a further asset of H2BC that the experiment is significantly shorter than HMBC and HSQC-TOCSY, and hence less sensitive to transverse relaxation. The H2BC experiment is demonstrated on an approximately 30-residue oligosaccharide from Francisella victoria. (c) 2005 Elsevier Ltd. All rights reserved.

Original language	English
Journal	Carbohydrate Research
Volume	341
Issue number	4
Pages (from-to)	550-556
Number of pages	7
ISSN	0008-6215
DOIs	https://doi.org/10.1016/j.carres.2005.11.020
Publication status	Published - 2006
Externally published	Yes

Keywords

Carbohydrates
Carbon Isotopes
Francisella
Hydrogen
Magnetic Resonance Spectroscopy
Oligosaccharides
Polysaccharides, Bacterial
7YNJ3PO35Z Hydrogen
Bond strength (chemical)
Carbon
Correlation methods
Demonstrations
Nuclear magnetic resonance
Oligomers
Protons
carbohydrate derivative
carbon
oligosaccharide
proton
article
bacterium
complex formation
correlation analysis
covalent bond
Francisella victoria
heteronuclear 2 bond correlation nuclear magnetic resonance
nuclear magnetic resonance
priority journal
technique
Bacteria (microorganisms)
13C
1H
H2BC
Oligosaccharide
NMR
T
BIOCHEMISTRY
CHEMISTRY,
COUPLING-CONSTANTS
SPECTRA
HOMONUCLEAR
H-1
C-13
Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Negative Aerobic Rods and Cocci [06500] Francisella victoria species
complex carbohydrates
oligosaccharides
10060, Biochemistry studies - General
10068, Biochemistry studies - Carbohydrates
31000, Physiology and biochemistry of bacteria
heteronuclear 2-bond correlation-nuclear magnetic resonance H2BC-NMR laboratory techniques, spectrum analysis techniques
Biochemistry and Molecular Biophysics
Methods and Techniques

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title = "H2BC: a new technique for NMR analysis of complex carbohydrates",

abstract = "It is demonstrated that the H2BC NMR pulse sequence (J Am. Chem. Soc. 2005, 127, 6154, Magn. Reson. Chem. 2005, 43, 971-974) offers unambiguous assignments and significant simplification of NMR spectra of large and complex carbohydrates compared to other techniques for the establishment of correlations over more than one bond. H2BC almost exclusively correlates protons and proton-bearing carbon spins separated by two covalent bonds and is independent of occasionally vanishing (2)J(CH) coupling constants, which alleviates the problem of missing two-bond correlations in HMBC spectra. H2BC also solves the problem of distinguishing two- and three-bond correlations in HSQC-TOCSY or HMBC. It is a further asset of H2BC that the experiment is significantly shorter than HMBC and HSQC-TOCSY, and hence less sensitive to transverse relaxation. The H2BC experiment is demonstrated on an approximately 30-residue oligosaccharide from Francisella victoria. (c) 2005 Elsevier Ltd. All rights reserved.",

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author = "Petersen, {Bent O.} and E. Vinogradov and W. Kay and P. Wurtz and NT Nyberg and Duus, {Jens {\O}llgaard} and OW Sorensen",

year = "2006",

doi = "10.1016/j.carres.2005.11.020",

language = "English",

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TY - JOUR

T1 - H2BC: a new technique for NMR analysis of complex carbohydrates

AU - Petersen, Bent O.

AU - Vinogradov, E.

AU - Kay, W.

AU - Wurtz, P.

AU - Nyberg, NT

AU - Duus, Jens Øllgaard

AU - Sorensen, OW

PY - 2006

Y1 - 2006

N2 - It is demonstrated that the H2BC NMR pulse sequence (J Am. Chem. Soc. 2005, 127, 6154, Magn. Reson. Chem. 2005, 43, 971-974) offers unambiguous assignments and significant simplification of NMR spectra of large and complex carbohydrates compared to other techniques for the establishment of correlations over more than one bond. H2BC almost exclusively correlates protons and proton-bearing carbon spins separated by two covalent bonds and is independent of occasionally vanishing (2)J(CH) coupling constants, which alleviates the problem of missing two-bond correlations in HMBC spectra. H2BC also solves the problem of distinguishing two- and three-bond correlations in HSQC-TOCSY or HMBC. It is a further asset of H2BC that the experiment is significantly shorter than HMBC and HSQC-TOCSY, and hence less sensitive to transverse relaxation. The H2BC experiment is demonstrated on an approximately 30-residue oligosaccharide from Francisella victoria. (c) 2005 Elsevier Ltd. All rights reserved.

AB - It is demonstrated that the H2BC NMR pulse sequence (J Am. Chem. Soc. 2005, 127, 6154, Magn. Reson. Chem. 2005, 43, 971-974) offers unambiguous assignments and significant simplification of NMR spectra of large and complex carbohydrates compared to other techniques for the establishment of correlations over more than one bond. H2BC almost exclusively correlates protons and proton-bearing carbon spins separated by two covalent bonds and is independent of occasionally vanishing (2)J(CH) coupling constants, which alleviates the problem of missing two-bond correlations in HMBC spectra. H2BC also solves the problem of distinguishing two- and three-bond correlations in HSQC-TOCSY or HMBC. It is a further asset of H2BC that the experiment is significantly shorter than HMBC and HSQC-TOCSY, and hence less sensitive to transverse relaxation. The H2BC experiment is demonstrated on an approximately 30-residue oligosaccharide from Francisella victoria. (c) 2005 Elsevier Ltd. All rights reserved.

KW - Carbohydrates

KW - Carbon Isotopes

KW - Francisella

KW - Hydrogen

KW - Magnetic Resonance Spectroscopy

KW - Oligosaccharides

KW - Polysaccharides, Bacterial

KW - 7YNJ3PO35Z Hydrogen

KW - Bond strength (chemical)

KW - Carbon

KW - Correlation methods

KW - Demonstrations

KW - Nuclear magnetic resonance

KW - Oligomers

KW - Protons

KW - carbohydrate derivative

KW - carbon

KW - oligosaccharide

KW - proton

KW - article

KW - bacterium

KW - complex formation

KW - correlation analysis

KW - covalent bond

KW - Francisella victoria

KW - heteronuclear 2 bond correlation nuclear magnetic resonance

KW - nuclear magnetic resonance

KW - priority journal

KW - technique

KW - Bacteria (microorganisms)

KW - 13C

KW - 1H

KW - H2BC

KW - Oligosaccharide

KW - NMR

KW - T

KW - BIOCHEMISTRY

KW - CHEMISTRY,

KW - COUPLING-CONSTANTS

KW - SPECTRA

KW - HOMONUCLEAR

KW - H-1

KW - C-13

KW - Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Negative Aerobic Rods and Cocci [06500] Francisella victoria species

KW - complex carbohydrates

KW - oligosaccharides

KW - 10060, Biochemistry studies - General

KW - 10068, Biochemistry studies - Carbohydrates

KW - 31000, Physiology and biochemistry of bacteria

KW - heteronuclear 2-bond correlation-nuclear magnetic resonance H2BC-NMR laboratory techniques, spectrum analysis techniques

KW - Biochemistry and Molecular Biophysics

KW - Methods and Techniques

U2 - 10.1016/j.carres.2005.11.020

DO - 10.1016/j.carres.2005.11.020

M3 - Journal article

C2 - 16406276

SN - 0008-6215

VL - 341

SP - 550

EP - 556

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 4

ER -

H2BC: a new technique for NMR analysis of complex carbohydrates

Abstract

Keywords

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