Growth of thin films of low molecular weight proteins by matrix assisted pulsed laser evaporation (MAPLE)

Andreea Matei, Jørgen Schou, C. Constantinescu, P. Kingshott, M. Dinescu

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Thin films of lysozyme and myoglobin grown by matrix assisted pulsed laser evaporation (MAPLE) from a water ice matrix have been investigated. The deposition rate of these two low molecular weight proteins (lysozyme: 14307 amu and myoglobin: 17083 amu) exhibits a maximum of about 1–2 ng/cm2 per pulse at a fluence of 1–2 J/cm2 and decreases slowly with increasing fluence. This rate is presumably determined by the matrix rather by the proteins. A significant fraction of the proteins are intact in the film as determined by MALDI (Matrix assisted laser desorption ionization) spectrometry. The results for lysozyme demonstrate that the fragmentation rate of the proteins during the MAPLE process is not influenced by the pH of the water solution prior to freezing.
Original languageEnglish
JournalApplied Physics A: Materials Science & Processing
Volume105
Issue number3
Pages (from-to)629-633
ISSN0947-8396
DOIs
Publication statusPublished - 2011

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