Growth and exopolysaccharide yield of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 in batch and continuous bioreactor experiments at constant pH

Susann Mende, Leona Krzyzanowski, Jost Weber, Doris Jaros*, Harald Rohm

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Some Lactobacillus delbrueckii ssp. bulgaricus strains are able to synthesize exopolysaccharides (EPS) and are therefore highly important for the dairy industry as starter cultures. The aim of this study was to investigate the nutritional requirements for growth and EPS production of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081. A medium was developed from a semi-defined medium (SDM) in which glucose was replaced by lactose and different combinations of supplements (nucleobases, vitamins, salts, sodium formate and orotic acid) were added. Constant pH batch fermentation with the modified medium resulted in an EPS yield of approximately 210. mg glucose equivalents per liter medium. This was a 10-fold increase over flask cultivation of this strain in SDM. Although not affecting cell growth, the mixture of salts enhanced the EPS synthesis. Whereas EPS production was approximately 12. mg/g dry biomass without salt supplementation, a significantly higher yield (approximately 20. mg/g dry biomass) was observed after adding the salt mixture. In continuous fermentation, a maximal EPS concentration was obtained at a dilution rate of 0.3. 1/h (80. mg EPS/L), which corresponded to a specific EPS production of 49. mg/g dry biomass.

Original languageEnglish
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number2
Pages (from-to)185-191
Number of pages7
ISSN1389-1723
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Batch fermentation
  • Broth supplementation
  • Continuous fermentation
  • Exopolysaccharide
  • Lactobacillus delbrueckii ssp. bulgaricus

Cite this

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title = "Growth and exopolysaccharide yield of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 in batch and continuous bioreactor experiments at constant pH",
abstract = "Some Lactobacillus delbrueckii ssp. bulgaricus strains are able to synthesize exopolysaccharides (EPS) and are therefore highly important for the dairy industry as starter cultures. The aim of this study was to investigate the nutritional requirements for growth and EPS production of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081. A medium was developed from a semi-defined medium (SDM) in which glucose was replaced by lactose and different combinations of supplements (nucleobases, vitamins, salts, sodium formate and orotic acid) were added. Constant pH batch fermentation with the modified medium resulted in an EPS yield of approximately 210. mg glucose equivalents per liter medium. This was a 10-fold increase over flask cultivation of this strain in SDM. Although not affecting cell growth, the mixture of salts enhanced the EPS synthesis. Whereas EPS production was approximately 12. mg/g dry biomass without salt supplementation, a significantly higher yield (approximately 20. mg/g dry biomass) was observed after adding the salt mixture. In continuous fermentation, a maximal EPS concentration was obtained at a dilution rate of 0.3. 1/h (80. mg EPS/L), which corresponded to a specific EPS production of 49. mg/g dry biomass.",
keywords = "Batch fermentation, Broth supplementation, Continuous fermentation, Exopolysaccharide, Lactobacillus delbrueckii ssp. bulgaricus",
author = "Susann Mende and Leona Krzyzanowski and Jost Weber and Doris Jaros and Harald Rohm",
year = "2012",
doi = "10.1016/j.jbiosc.2011.10.012",
language = "English",
volume = "113",
pages = "185--191",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "Society for Biotechnology, Japan",
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}

Growth and exopolysaccharide yield of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 in batch and continuous bioreactor experiments at constant pH. / Mende, Susann; Krzyzanowski, Leona; Weber, Jost; Jaros, Doris; Rohm, Harald.

In: Journal of Bioscience and Bioengineering, Vol. 113, No. 2, 2012, p. 185-191.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Growth and exopolysaccharide yield of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 in batch and continuous bioreactor experiments at constant pH

AU - Mende, Susann

AU - Krzyzanowski, Leona

AU - Weber, Jost

AU - Jaros, Doris

AU - Rohm, Harald

PY - 2012

Y1 - 2012

N2 - Some Lactobacillus delbrueckii ssp. bulgaricus strains are able to synthesize exopolysaccharides (EPS) and are therefore highly important for the dairy industry as starter cultures. The aim of this study was to investigate the nutritional requirements for growth and EPS production of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081. A medium was developed from a semi-defined medium (SDM) in which glucose was replaced by lactose and different combinations of supplements (nucleobases, vitamins, salts, sodium formate and orotic acid) were added. Constant pH batch fermentation with the modified medium resulted in an EPS yield of approximately 210. mg glucose equivalents per liter medium. This was a 10-fold increase over flask cultivation of this strain in SDM. Although not affecting cell growth, the mixture of salts enhanced the EPS synthesis. Whereas EPS production was approximately 12. mg/g dry biomass without salt supplementation, a significantly higher yield (approximately 20. mg/g dry biomass) was observed after adding the salt mixture. In continuous fermentation, a maximal EPS concentration was obtained at a dilution rate of 0.3. 1/h (80. mg EPS/L), which corresponded to a specific EPS production of 49. mg/g dry biomass.

AB - Some Lactobacillus delbrueckii ssp. bulgaricus strains are able to synthesize exopolysaccharides (EPS) and are therefore highly important for the dairy industry as starter cultures. The aim of this study was to investigate the nutritional requirements for growth and EPS production of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081. A medium was developed from a semi-defined medium (SDM) in which glucose was replaced by lactose and different combinations of supplements (nucleobases, vitamins, salts, sodium formate and orotic acid) were added. Constant pH batch fermentation with the modified medium resulted in an EPS yield of approximately 210. mg glucose equivalents per liter medium. This was a 10-fold increase over flask cultivation of this strain in SDM. Although not affecting cell growth, the mixture of salts enhanced the EPS synthesis. Whereas EPS production was approximately 12. mg/g dry biomass without salt supplementation, a significantly higher yield (approximately 20. mg/g dry biomass) was observed after adding the salt mixture. In continuous fermentation, a maximal EPS concentration was obtained at a dilution rate of 0.3. 1/h (80. mg EPS/L), which corresponded to a specific EPS production of 49. mg/g dry biomass.

KW - Batch fermentation

KW - Broth supplementation

KW - Continuous fermentation

KW - Exopolysaccharide

KW - Lactobacillus delbrueckii ssp. bulgaricus

U2 - 10.1016/j.jbiosc.2011.10.012

DO - 10.1016/j.jbiosc.2011.10.012

M3 - Journal article

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VL - 113

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