Growing and Analyzing Biofilms in Flow Chambers

Tim Tolker-Nielsen, Claus Sternberg

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    This unit describes the setup of flow chamber systems for the study of microbial biofilms, and methods for the analysis of structural biofilm formation. Use of flow chambers allows direct microscopic investigation of biofilm formation. The biofilms in flow chambers develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow chamber and the bubble trap, assembly and sterilization of the flow chamber system, inoculation of the flow chambers, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow chamber–grown biofilms are addressed. Curr. Protoc. Microbiol. 21:1B.2.1-1B.2.17. © 2011 by John Wiley & Sons, Inc.
    Original languageEnglish
    Title of host publicationCurrent Protocols in Microbiology
    Volume1B.2
    PublisherJohn Wiley & Sons Ltd
    Publication date2011
    ISBN (Electronic)9780471729259
    Publication statusPublished - 2011

    Keywords

    • Comstat
    • Flow chamber
    • Biofilm
    • Fluorescent in situ hybridization
    • Confocal laser scanning microscopy
    • Bubble trap

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