Green synthesis of silver nanoparticles using acacia lignin, their cytotoxicity, catalytic, metal ion sensing capability and antibacterial activity

Keshaw R. Aadil, Neha Pandey, Solange I. Mussatto, Harit Jha

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The present study uncovers the in-vitro cytotoxicity, sensing properties, catalytic and antibacterial activity of lignin functionalized silver nanoparticles (AGNPs). Fabricated AGNPs were characterized using UV-vis spectroscopy, particle size, X-ray diffraction (XRD) and Transmission Electron Microscopic (TEM) analyses. The typical surface plasmon resonance peak of AGNP was recorded at 410 nm, as confirmed from UV-vis spectrum. Synthesized AGNPs displayed spherical shape and face centric cubic structure with average size of 10-50 nm. In-vitro cytotoxicity assay using MCF-7 cancer cell line revealed that the extracted lignin was non-toxic while AGNPs displayed toxicity at both 5 and 50 μg/ml concentrations, respectively. Evaluation of the sensing property of AGNPs suggested that the lignin stabilized silver nanoparticles have potential to detect the concentration of heavy metal ions up to nanomolar range. AGNPs also showed good catalytic activity with the rate constants of 0.00098 and 0.01525 min-1 respectively for methylene blue dye and p-nitrophenol reduction. Moreover, AGNPs displayed antibacterial efficacy against both gram positive and negative strains. These findings suggest that lignin mediated AGNPs can find applications in different fields including biomedical, drug delivery, textile, biosensor, food packaging material, and textiles.
Original languageEnglish
Article number103296
JournalJournal of Environmental Chemical Engineering
Volume7
Issue number5
ISSN2213-3437
DOIs
Publication statusPublished - 2019

Keywords

  • Lignin
  • Silver nanoparticles
  • in-Vitro cytotoxicity
  • Metal ion sensing properties
  • Catalytic activity
  • Antimicrobial activity

Cite this

@article{559e63eca1e04f2b8eb1d05ec55bcc50,
title = "Green synthesis of silver nanoparticles using acacia lignin, their cytotoxicity, catalytic, metal ion sensing capability and antibacterial activity",
abstract = "The present study uncovers the in-vitro cytotoxicity, sensing properties, catalytic and antibacterial activity of lignin functionalized silver nanoparticles (AGNPs). Fabricated AGNPs were characterized using UV-vis spectroscopy, particle size, X-ray diffraction (XRD) and Transmission Electron Microscopic (TEM) analyses. The typical surface plasmon resonance peak of AGNP was recorded at 410 nm, as confirmed from UV-vis spectrum. Synthesized AGNPs displayed spherical shape and face centric cubic structure with average size of 10-50 nm. In-vitro cytotoxicity assay using MCF-7 cancer cell line revealed that the extracted lignin was non-toxic while AGNPs displayed toxicity at both 5 and 50 μg/ml concentrations, respectively. Evaluation of the sensing property of AGNPs suggested that the lignin stabilized silver nanoparticles have potential to detect the concentration of heavy metal ions up to nanomolar range. AGNPs also showed good catalytic activity with the rate constants of 0.00098 and 0.01525 min-1 respectively for methylene blue dye and p-nitrophenol reduction. Moreover, AGNPs displayed antibacterial efficacy against both gram positive and negative strains. These findings suggest that lignin mediated AGNPs can find applications in different fields including biomedical, drug delivery, textile, biosensor, food packaging material, and textiles.",
keywords = "Lignin, Silver nanoparticles, in-Vitro cytotoxicity, Metal ion sensing properties, Catalytic activity, Antimicrobial activity",
author = "Aadil, {Keshaw R.} and Neha Pandey and Mussatto, {Solange I.} and Harit Jha",
year = "2019",
doi = "10.1016/j.jece.2019.103296",
language = "English",
volume = "7",
journal = "Journal of Environmental Chemical Engineering",
issn = "2213-3437",
publisher = "Elsevier",
number = "5",

}

Green synthesis of silver nanoparticles using acacia lignin, their cytotoxicity, catalytic, metal ion sensing capability and antibacterial activity. / Aadil, Keshaw R.; Pandey, Neha; Mussatto, Solange I.; Jha, Harit.

In: Journal of Environmental Chemical Engineering, Vol. 7, No. 5, 103296, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Green synthesis of silver nanoparticles using acacia lignin, their cytotoxicity, catalytic, metal ion sensing capability and antibacterial activity

AU - Aadil, Keshaw R.

AU - Pandey, Neha

AU - Mussatto, Solange I.

AU - Jha, Harit

PY - 2019

Y1 - 2019

N2 - The present study uncovers the in-vitro cytotoxicity, sensing properties, catalytic and antibacterial activity of lignin functionalized silver nanoparticles (AGNPs). Fabricated AGNPs were characterized using UV-vis spectroscopy, particle size, X-ray diffraction (XRD) and Transmission Electron Microscopic (TEM) analyses. The typical surface plasmon resonance peak of AGNP was recorded at 410 nm, as confirmed from UV-vis spectrum. Synthesized AGNPs displayed spherical shape and face centric cubic structure with average size of 10-50 nm. In-vitro cytotoxicity assay using MCF-7 cancer cell line revealed that the extracted lignin was non-toxic while AGNPs displayed toxicity at both 5 and 50 μg/ml concentrations, respectively. Evaluation of the sensing property of AGNPs suggested that the lignin stabilized silver nanoparticles have potential to detect the concentration of heavy metal ions up to nanomolar range. AGNPs also showed good catalytic activity with the rate constants of 0.00098 and 0.01525 min-1 respectively for methylene blue dye and p-nitrophenol reduction. Moreover, AGNPs displayed antibacterial efficacy against both gram positive and negative strains. These findings suggest that lignin mediated AGNPs can find applications in different fields including biomedical, drug delivery, textile, biosensor, food packaging material, and textiles.

AB - The present study uncovers the in-vitro cytotoxicity, sensing properties, catalytic and antibacterial activity of lignin functionalized silver nanoparticles (AGNPs). Fabricated AGNPs were characterized using UV-vis spectroscopy, particle size, X-ray diffraction (XRD) and Transmission Electron Microscopic (TEM) analyses. The typical surface plasmon resonance peak of AGNP was recorded at 410 nm, as confirmed from UV-vis spectrum. Synthesized AGNPs displayed spherical shape and face centric cubic structure with average size of 10-50 nm. In-vitro cytotoxicity assay using MCF-7 cancer cell line revealed that the extracted lignin was non-toxic while AGNPs displayed toxicity at both 5 and 50 μg/ml concentrations, respectively. Evaluation of the sensing property of AGNPs suggested that the lignin stabilized silver nanoparticles have potential to detect the concentration of heavy metal ions up to nanomolar range. AGNPs also showed good catalytic activity with the rate constants of 0.00098 and 0.01525 min-1 respectively for methylene blue dye and p-nitrophenol reduction. Moreover, AGNPs displayed antibacterial efficacy against both gram positive and negative strains. These findings suggest that lignin mediated AGNPs can find applications in different fields including biomedical, drug delivery, textile, biosensor, food packaging material, and textiles.

KW - Lignin

KW - Silver nanoparticles

KW - in-Vitro cytotoxicity

KW - Metal ion sensing properties

KW - Catalytic activity

KW - Antimicrobial activity

U2 - 10.1016/j.jece.2019.103296

DO - 10.1016/j.jece.2019.103296

M3 - Journal article

VL - 7

JO - Journal of Environmental Chemical Engineering

JF - Journal of Environmental Chemical Engineering

SN - 2213-3437

IS - 5

M1 - 103296

ER -