Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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  • Author: Amato, Alberto

    Stazione Zoologica Anton Dohrn Napoli, Italy

  • Author: Sabatino, Valeria

    Stazione Zoologica Anton Dohrn Napoli, Italy

  • Author: Nylund, Göran M.

    University of Gothenburg, Sweden

  • Author: Bergkvist, Johanna

    University of Gothenburg, Sweden

  • Author: Basu, Swaraj

    Stazione Zoologica Anton Dohrn Napoli, Italy

  • Author: Andersson, Mats X.

    University of Gothenburg, Sweden

  • Author: Sanges, Remo

    Stazione Zoologica Anton Dohrn Napoli, Italy

  • Author: Godhe, Anna

    University of Gothenburg, Sweden

  • Author: Kiørboe, Thomas

    Centre for Ocean Life, National Institute of Aquatic Resources, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Selander, Erik

    University of Gothenburg, Sweden

  • Author: Ferrante, Maria I.

    Stazione Zoologica Anton Dohrn Napoli, Italy

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Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems.
Original languageEnglish
JournalI S M E Journal
Volume12
Pages (from-to)1594-1604
ISSN1751-7362
DOIs
Publication statusPublished - 2018
CitationsWeb of Science® Times Cited: No match on DOI

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