Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi

Alberto Amato, Valeria Sabatino, Göran M. Nylund, Johanna Bergkvist, Swaraj Basu, Mats X. Andersson, Remo Sanges, Anna Godhe, Thomas Kiørboe, Erik Selander, Maria I. Ferrante*

*Corresponding author for this work

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Abstract

Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems.
Original languageEnglish
JournalI S M E Journal
Volume12
Pages (from-to)1594-1604
ISSN1751-7362
DOIs
Publication statusPublished - 2018

Cite this

Amato, A., Sabatino, V., Nylund, G. M., Bergkvist, J., Basu, S., Andersson, M. X., ... Ferrante, M. I. (2018). Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi. I S M E Journal, 12, 1594-1604. https://doi.org/10.1038/s41396-018-0094-0
Amato, Alberto ; Sabatino, Valeria ; Nylund, Göran M. ; Bergkvist, Johanna ; Basu, Swaraj ; Andersson, Mats X. ; Sanges, Remo ; Godhe, Anna ; Kiørboe, Thomas ; Selander, Erik ; Ferrante, Maria I. / Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi. In: I S M E Journal. 2018 ; Vol. 12. pp. 1594-1604.
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title = "Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi",
abstract = "Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems.",
author = "Alberto Amato and Valeria Sabatino and Nylund, {G{\"o}ran M.} and Johanna Bergkvist and Swaraj Basu and Andersson, {Mats X.} and Remo Sanges and Anna Godhe and Thomas Ki{\o}rboe and Erik Selander and Ferrante, {Maria I.}",
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language = "English",
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Amato, A, Sabatino, V, Nylund, GM, Bergkvist, J, Basu, S, Andersson, MX, Sanges, R, Godhe, A, Kiørboe, T, Selander, E & Ferrante, MI 2018, 'Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi', I S M E Journal, vol. 12, pp. 1594-1604. https://doi.org/10.1038/s41396-018-0094-0

Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi. / Amato, Alberto; Sabatino, Valeria; Nylund, Göran M.; Bergkvist, Johanna; Basu, Swaraj; Andersson, Mats X.; Sanges, Remo; Godhe, Anna; Kiørboe, Thomas; Selander, Erik; Ferrante, Maria I.

In: I S M E Journal, Vol. 12, 2018, p. 1594-1604.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi

AU - Amato, Alberto

AU - Sabatino, Valeria

AU - Nylund, Göran M.

AU - Bergkvist, Johanna

AU - Basu, Swaraj

AU - Andersson, Mats X.

AU - Sanges, Remo

AU - Godhe, Anna

AU - Kiørboe, Thomas

AU - Selander, Erik

AU - Ferrante, Maria I.

PY - 2018

Y1 - 2018

N2 - Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems.

AB - Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems.

U2 - 10.1038/s41396-018-0094-0

DO - 10.1038/s41396-018-0094-0

M3 - Journal article

VL - 12

SP - 1594

EP - 1604

JO - I S M E Journal

JF - I S M E Journal

SN - 1751-7362

ER -