Graphene Functionalized Decellularized Scaffold Promotes Skin Cell Proliferation

Mahboubeh Jafarkhani, Zeinab Salehi*, Zahra Bagheri, Zahra Aayanifard, Ali Rezvan, Hamid Doosthosseini, Mohammad Ali Shokrgozar

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

An increasing number of new strategies for skin tissue engineering have been developed with the potential to mimic the biological properties of native tissue with a high degree of complexity, flexibility, and reproducibility. In this study, decellularized tissue (DT) was prepared from the bovine heart by using chemical treatments. However, the mechanical properties of the DT constructs were poorer than the extra cellular matrix of the skin tissue. To overcome this challenge, hybrid scaffolds of DT and graphene oxide (GO) were developed and the effects of the GO concentration on the morphology, pore size, porosity, mechanical strength, and water uptake capacity of the samples were evaluated. Moreover, the biocompatibility of hybrid scaffolds was studied by Live/Dead staining. The results show that a hybrid scaffold incorporating 3 % graphene oxide improved the mechanical strength and cell viability by ~25 % in comparison to the DT scaffolds. Cell viability results confirmed that the porous scaffolds could support cell adhesion, proliferation, and cell activity for 7 days. This study provides new insight into and opportunities for using graphene‐based materials to develop biomimetic constructs for clinical applications.
Original languageEnglish
JournalCanadian Journal of Chemical Engineering
Volume98
Issue number1
Pages (from-to)62-68
ISSN0008-4034
DOIs
Publication statusPublished - 2020

Keywords

  • Decellularized tissues
  • Biomimetic scaffolds
  • Graphene oxide
  • Skin tissue engineering

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