Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

Johanna Noireaux, Romain Grall, Marie Hullo, Sylvie Chevillard, Caroline Oster, Emilie Brun, Cécile Sicard-Roselli, Katrin Löschner, Paola Fisicaro*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Gold nanoparticles (AuNPs) are increasingly studied for cancer treatment purposes, as they can potentially improve both control and efficiency of the treatment. Intensive research is conducted in vitro on rodent and human cell lines to objectify the gain of combining AuNPs with cancer treatment and to understand their mechanisms of action. However, using nanoparticles in such studies requires thorough knowledge of their cellular uptake. In this study, we optimized single particle ICPMS (sp-ICPMS) analysis to qualify and quantify intracellular AuNP content after exposure of in vitro human breast cancer cell lines. To this aim, cells were treated with an alkaline digestion method with 5% TMAH, allowing the detection of gold with a yield of 97% on average. Results showed that under our experimental conditions, the AuNP size distribution appeared to be unchanged after internalization and that the uptake of particles depended on the cell line and on the exposure duration. Finally, the comparison of the particle numbers per cell with the estimates based on the gold masses showed excellent agreement, confirming the validity of the sp-ICPMS particle measurements in such complex samples.
Original languageEnglish
JournalSeparations
Volume6
Issue number3
Number of pages13
ISSN2297-8739
DOIs
Publication statusPublished - 2019

Keywords

  • sp-ICPMS
  • gold nanoparticles
  • tumor cells
  • nanoparticle uptake
  • Physics
  • QC1-999
  • Chemistry
  • QD1-999

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