Gold-based optical biosensor for single-mismatched DNA detection using salt-induced hybridization

Zongrui Zhan; Xingyi Ma; Cuong Cao; Sang Jun Sim

doi:10.1016/j.bios.2011.11.045

Gold-based optical biosensor for single-mismatched DNA detection using salt-induced hybridization

Zongrui Zhan, Xingyi Ma, Cuong Cao, Sang Jun Sim

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

In this study, a gold nanoparticle (Au-NP)-based detection method for sensitive and specific DNA-based diagnostic applications is described. A sandwich format consisting of Au-NPs/DNA/PMP (Streptavidin-coated MagnetSphere Para-Magnetic Particles) was fabricated. PMPs captured and separated target DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which could easily and quantitatively measured by low-cost UV–vis spectrophotometric analysis. The results indicated this to be a very simple and economic strategy for detection of single-mismatched DNA strands.

Original language	English
Journal	Biosensors and Bioelectronics
Volume	32
Issue number	12
Pages (from-to)	127-132
ISSN	0956-5663
DOIs	https://doi.org/10.1016/j.bios.2011.11.045
Publication status	Published - 2011

Keywords

DNA hybridization
Salt-induced
BRCA-1
Single-mismatched detection
Gold nanoparticles

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title = "Gold-based optical biosensor for single-mismatched DNA detection using salt-induced hybridization",

abstract = "In this study, a gold nanoparticle (Au-NP)-based detection method for sensitive and specific DNA-based diagnostic applications is described. A sandwich format consisting of Au-NPs/DNA/PMP (Streptavidin-coated MagnetSphere Para-Magnetic Particles) was fabricated. PMPs captured and separated target DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which could easily and quantitatively measured by low-cost UV–vis spectrophotometric analysis. The results indicated this to be a very simple and economic strategy for detection of single-mismatched DNA strands.",

keywords = "DNA hybridization, Salt-induced, BRCA-1, Single-mismatched detection, Gold nanoparticles",

author = "Zongrui Zhan and Xingyi Ma and Cuong Cao and Sim, {Sang Jun}",

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AU - Zhan, Zongrui

AU - Ma, Xingyi

AU - Cao, Cuong

AU - Sim, Sang Jun

PY - 2011

Y1 - 2011

N2 - In this study, a gold nanoparticle (Au-NP)-based detection method for sensitive and specific DNA-based diagnostic applications is described. A sandwich format consisting of Au-NPs/DNA/PMP (Streptavidin-coated MagnetSphere Para-Magnetic Particles) was fabricated. PMPs captured and separated target DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which could easily and quantitatively measured by low-cost UV–vis spectrophotometric analysis. The results indicated this to be a very simple and economic strategy for detection of single-mismatched DNA strands.

AB - In this study, a gold nanoparticle (Au-NP)-based detection method for sensitive and specific DNA-based diagnostic applications is described. A sandwich format consisting of Au-NPs/DNA/PMP (Streptavidin-coated MagnetSphere Para-Magnetic Particles) was fabricated. PMPs captured and separated target DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which could easily and quantitatively measured by low-cost UV–vis spectrophotometric analysis. The results indicated this to be a very simple and economic strategy for detection of single-mismatched DNA strands.

KW - DNA hybridization

KW - Salt-induced

KW - BRCA-1

KW - Single-mismatched detection

KW - Gold nanoparticles

U2 - 10.1016/j.bios.2011.11.045

DO - 10.1016/j.bios.2011.11.045

M3 - Journal article

C2 - 22188920

SN - 0956-5663

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EP - 132

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 12

ER -

Gold-based optical biosensor for single-mismatched DNA detection using salt-induced hybridization

Abstract

Keywords

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