Gold-based optical biosensor for single-mismatched DNA detection using salt-induced hybridization

Zongrui Zhan, Xingyi Ma, Cuong Cao, Sang Jun Sim

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In this study, a gold nanoparticle (Au-NP)-based detection method for sensitive and specific DNA-based diagnostic applications is described. A sandwich format consisting of Au-NPs/DNA/PMP (Streptavidin-coated MagnetSphere Para-Magnetic Particles) was fabricated. PMPs captured and separated target DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which could easily and quantitatively measured by low-cost UV–vis spectrophotometric analysis. The results indicated this to be a very simple and economic strategy for detection of single-mismatched DNA strands.
    Original languageEnglish
    JournalBiosensors and Bioelectronics
    Volume32
    Issue number12
    Pages (from-to)127-132
    ISSN0956-5663
    DOIs
    Publication statusPublished - 2011

    Keywords

    • DNA hybridization
    • Salt-induced
    • BRCA-1
    • Single-mismatched detection
    • Gold nanoparticles

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