Glucosinolate engineering identifies a gamma-glutamyl peptidase.

Fernando Geu-Flores; Morten Thrane Nielsen; Majse Nafisi; Morten Emil Møldrup; Carl Erik Olsen; Mohammed Saddik Motawia; Barbara Ann Halkier

doi:10.1038/nchembio.185

Glucosinolate engineering identifies a gamma-glutamyl peptidase.

Fernando Geu-Flores
, Morten Thrane Nielsen
, Majse Nafisi
, Morten Emil Møldrup
, Carl Erik Olsen
, Mohammed Saddik Motawia
, Barbara Ann Halkier

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.

Original language	English
Journal	Nature Chemical Biology
Volume	5
Issue number	8
Pages (from-to)	575-577
ISSN	1552-4450
DOIs	https://doi.org/10.1038/nchembio.185
Publication status	Published - 2009
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1038/nchembio.185

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title = "Glucosinolate engineering identifies a gamma-glutamyl peptidase.",

abstract = "Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.",

author = "Fernando Geu-Flores and Nielsen, \{Morten Thrane\} and Majse Nafisi and M{\o}ldrup, \{Morten Emil\} and Olsen, \{Carl Erik\} and Motawia, \{Mohammed Saddik\} and Halkier, \{Barbara Ann\}",

year = "2009",

doi = "10.1038/nchembio.185",

language = "English",

volume = "5",

pages = "575--577",

journal = "Nature Chemical Biology",

issn = "1552-4450",

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TY - JOUR

T1 - Glucosinolate engineering identifies a gamma-glutamyl peptidase.

AU - Geu-Flores, Fernando

AU - Nielsen, Morten Thrane

AU - Nafisi, Majse

AU - Møldrup, Morten Emil

AU - Olsen, Carl Erik

AU - Motawia, Mohammed Saddik

AU - Halkier, Barbara Ann

PY - 2009

Y1 - 2009

N2 - Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.

AB - Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.

U2 - 10.1038/nchembio.185

DO - 10.1038/nchembio.185

M3 - Journal article

SN - 1552-4450

VL - 5

SP - 575

EP - 577

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 8

ER -

Glucosinolate engineering identifies a gamma-glutamyl peptidase.

Abstract

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