TY - JOUR
T1 - Glucosinolate engineering identifies a gamma-glutamyl peptidase.
AU - Geu-Flores, Fernando
AU - Nielsen, Morten Thrane
AU - Nafisi, Majse
AU - Møldrup, Morten Emil
AU - Olsen, Carl Erik
AU - Motawia, Mohammed Saddik
AU - Halkier, Barbara Ann
PY - 2009
Y1 - 2009
N2 - Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.
AB - Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.
U2 - 10.1038/nchembio.185
DO - 10.1038/nchembio.185
M3 - Journal article
SN - 1552-4450
VL - 5
SP - 575
EP - 577
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 8
ER -
