Glucose and maltose metabolism in MIG1-disrupted and MAL-constitutive strains of Saccharomyces cerevisiae

Christopher Klein, Lisbeth Olsson, B Rønnow, Jørn Dalgaard Mikkelsen, Jens Bredal Nielsen

Research output: Contribution to journalConference articleResearchpeer-review

Abstract

The alleviation of glucose control of maltose metabolism brought about by MIG1 disruption was compared to that by MAL overexpression in a haploid Saccharomyces cerevisiae strain. The sugar consumption profiles during cultivation of the wild type, single transformants and a double transformant in a mixed glucose-maltose medium revealed that the MAL-constitutive strains were more alleviated than the single MIG1-disrupted transformant. While all transformants exhibited higher maximum specific growth rates (0.24-0.25 h(-1)) in glucose-maltose mixtures than the wild type strain (0.20 h(-1)), the MAL-constitutive transformants grew even faster (0.27-0.30 h(-1)) in pure glucose medium than the wild type strain (0.24 h(-1)).
Original languageEnglish
JournalFood Technology and Biotechnology
Volume35
Issue number4
Pages (from-to)287-292
ISSN1330-9862
Publication statusPublished - 1997
EventMeeting on Yeast Nutrition and Natural Habitats at the 18th International Specialised Symposium on Yeast (ISSY) - Bled, Slovenia
Duration: 24 Aug 199729 Aug 1997
Conference number: 18

Conference

ConferenceMeeting on Yeast Nutrition and Natural Habitats at the 18th International Specialised Symposium on Yeast (ISSY)
Number18
CountrySlovenia
CityBled
Period24/08/199729/08/1997

Keywords

  • Saccharomyces cerevisiae
  • baker's yeast
  • glucose repression
  • catabolite inactivation
  • MIG1
  • MAL
  • genetic engineering

Cite this

Klein, C., Olsson, L., Rønnow, B., Mikkelsen, J. D., & Nielsen, J. B. (1997). Glucose and maltose metabolism in MIG1-disrupted and MAL-constitutive strains of Saccharomyces cerevisiae. Food Technology and Biotechnology, 35(4), 287-292.