Gliadin affects glucose homeostasis and intestinal metagenome in C57BL6 mice fed a high-fat diet

Li Zhang, Axel Kornerup Hansen, Martin Iain Bahl, Camilla Hartmann Friis Hansen, Daniel Andersen, Susanne Brix Pedersen, Lars Hellgren, Tine Rask Licht

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Abstract

Dietary gluten and its component gliadin are well-known environmental triggers of celiac disease and important actors in type-1 diabetes, and are reported to induce alterations in the intestinal microbiota. However, research on the impact of gluten on type-2 diabetes in non-celiac subjects is more limited. The aim of this study was to investigate the effect of gliadin on glucose homeostasis and intestinal ecology in the mouse.

Forty male C57BL/6 mice were fed a high-fat diet containing either 4% gliadin or no gliadin for 22 weeks. Gliadin consumption significantly increased the HbA1c level over time, with a borderline significance of higher HOMA-IR (homeostasis model assessment of insulin resistance) after 22 weeks. Sequencing of the V3 region of the bacterial 16S rRNA genes showed that gliadin altered the abundance of 81 bacterial taxa, separating the intestinal microbial profile of the gliadin consuming mice from the control mice in the principal coordinate analysis (PCoA) of weighted UniFrac distance. Moreover, gliadin reduced the ileal gene expression of tight junction protein 1, occludin, cadherin 1, mucin 2 and mucin 3, indicating an impaired intestinal barrier function. No difference was found in body weight gain, feed consumption or circulating cytokines (IL-1β, IL-6, IFN-γ, TNF-α and IL-10).

Our study is the first to show that gliadin as part of a defined synthetic feed exacerbates the glycaemia and alters the intestinal microbiota composition. Comprehensive analyses of metabolites, histological sections and the profile of specific immune cells are in progress to elucidate the mechanism behind the observed effects.
Original languageEnglish
Publication date2015
Number of pages1
Publication statusPublished - 2015
EventKeystone Symposia - Gut Microbiota Modulation of Host Physiology: The Search for Mechanism - Keystone, Colorado, United States
Duration: 1 Mar 20156 Mar 2015

Conference

ConferenceKeystone Symposia - Gut Microbiota Modulation of Host Physiology: The Search for Mechanism
CountryUnited States
CityKeystone, Colorado
Period01/03/201506/03/2015

Cite this

Zhang, L., Hansen, A. K., Bahl, M. I., Hansen, C. H. F., Andersen, D., Pedersen, S. B., Hellgren, L., & Licht, T. R. (2015). Gliadin affects glucose homeostasis and intestinal metagenome in C57BL6 mice fed a high-fat diet. Abstract from Keystone Symposia - Gut Microbiota Modulation of Host Physiology: The Search for Mechanism, Keystone, Colorado, United States.