Gliadin affects glucose homeostasis and intestinal metagenome in C57BL/6 mice fed and high-fat diet

Li Zhang, Axel Kornerup Hansen, Martin Iain Bahl, Camilla Hartmann Friis Hansen, Daniel Andersen, Susanne Brix Pedersen, Lars Hellgren, Tine Rask Licht

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Abstract

Dietary gluten and its component gliadin are well-known environmental triggers of celiac disease and important actors in type-1 diabetes, and are reported to induce alterations in the intestinal microbiota. However, research on the impact of gluten on type-2 diabetes in non-celiac subjects is more limited. The aim of this study was to investigate the effect of gliadin on glucose homeostasis and intestinal ecology in the mouse. Forty male C57BL/6 mice were fed a high-fat diet containing either 4% gliadin or no gliadin for 22 weeks. Gliadin consumption significantly increased the HbA1c level over time, with a borderline significance of higher HOMA-IR (homeostasis model assessment of insulin resistance) after 22 weeks. Sequencing of the V3 region of the bacterial 16S rRNA genes showed that gliadin changed the abundance of 81 bacterial taxa, separating the intestinal microbial profile of the gliadin consuming mice from the control mice in the principal coordinate analysis (PCoA) of weighted UniFrac distance. No difference was found in body weight gain, feed consumption or circulating cytokines (IL-1β, IL-6, IFN-γ, TNF-α and IL-10). Our study is the first to show that gliadin as part of a defined synthetic feed exacerbates the glycaemia and alters the intestinal microbiota composition. Comprehensive analyses of the profile of specific immune cells, metabolites and intestinal permeability are in progress to elucidate the mechanism behind the observed effects.
Original languageEnglish
Publication date2014
Number of pages1
Publication statusPublished - 2014
EventThe Danish Microbiological Society Annual Congress 2014 - Eigtved's Pakhus, Copenhagen, Denmark
Duration: 10 Nov 2014 → …

Conference

ConferenceThe Danish Microbiological Society Annual Congress 2014
LocationEigtved's Pakhus
CountryDenmark
CityCopenhagen
Period10/11/2014 → …

Activities

  • 1 Conference presentations

Gliadin Affects Glucose Homeostasis and Intestinal Metagenome in C57BL/6 Mice Fed a High-Fat Diet

Li Zhang (Speaker)

10 Nov 2014

Activity: Talks and presentationsConference presentations

Cite this

Zhang, L., Hansen, A. K., Bahl, M. I., Hansen, C. H. F., Andersen, D., Pedersen, S. B., Hellgren, L., & Licht, T. R. (2014). Gliadin affects glucose homeostasis and intestinal metagenome in C57BL/6 mice fed and high-fat diet. Abstract from The Danish Microbiological Society Annual Congress 2014, Copenhagen, Denmark.