Giemsa C-banding of Barley Chromosomes. III. Segregation and Linkage of C-bands on Chromosomes 3, 6, and 7

Sixty-five homozygous barley lines, i.e. coming from chromosome-doubled monoploids derived from female gametes of F1 plants by the bulbosum method, segregated as expected in accordance with a 1:1-ratio for C-bands at two locations on chromosome 3 and at one location on chromosome 6. C-bands at one location on chromosome 7 segregated in a deviating ratio. Genes in three loci, Estl, M1-a9, and Amyl, on chromosomes 3, 5 and 6, respectively, segregated as expected in accordance with a 1:1-ratio. The two band locations on chromosome 3, which are separated cytologically by about one-fourth the arm length, showed no recombination; no linkage was detected between the two band locations and the Estl locus. The band location on chromosome 6 and the Amyl locus were linked with a recombination percentage of 26±5.5. It is safe to assume that some, if not all, C-bands are inherited as unit characters and can be used as markers in cytogenetic studies.