gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

Jens Bo Andersen, Arne Heydorn, Morten Hentzer, Leo Eberl, Otto Geisenberger, Bjarke Bak Christensen, Søren Molin, Michael Christian Givskov

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P-luxI have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the art tool for studies of communication between the individuals present in mixed bacterial communities.
    Original languageEnglish
    JournalApplied and Environmental Microbiology
    Volume67
    Issue number2
    Pages (from-to)575-585
    ISSN0099-2240
    Publication statusPublished - 2001

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