Germ cell numbers in human embryonic and fetal gonads during the first two trimesters of pregnancy

Analysis of six published studies

L. Salto Mamsen, M.C. Lutterodt, Elisabeth Wreford Andersen, A.G. Byskov, C. Yding Andersen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

BACKGROUND: The number of germ cells in human embryonic and fetal ovaries in relation to age is currently based on volumetric estimations from one study including a total of 12 ovaries. Six recent publications present stereological estimations of the number of germ cells in ovaries and testes for the first two trimesters. ; METHODS: Germ cell numbers from 103 human first and second trimester gonads aged 37–133 days post-conception (p.c.), obtained after legal termination of pregnancy, were collected from six independent studies that all used similar validated stereological methods for estimating germ cell numbers as well as somatic cell numbers. ; RESULTS: Statistically, the six studies estimated similar number of germ cells (P > 0.05) and no interaction between the studies and age was found (P > 0.05), indicating that the increase in cell numbers in relation to age was of comparable magnitude in each study. The number of germ cells increased from a mean of 7200 to 4 933 000 in fetal ovaries and from 3700 to 1 417 000 in fetal testes, from week 5 to week 19 p.c. A higher rate of increase was found for female germ cells as compared with males (P = 0.004). During the same period, the number of somatic cells increased from a mean of 158 000 to 1 017 000 in ovaries and from 154 000 to 2 035 000 in testes, respectively. ; CONCLUSIONS: By the use of validated stereological methods, this study provides more accurate and improved information on human germ and somatic cell numbers in ovaries and testes during the first two trimesters of pregnancy.
Original languageEnglish
JournalHuman Reproduction
Volume26
Issue number8
Pages (from-to)2140-2145
ISSN0268-1161
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Germ cells
  • Human embryos
  • First and second trimester pregnancy

Cite this

Mamsen, L. Salto ; Lutterodt, M.C. ; Andersen, Elisabeth Wreford ; Byskov, A.G. ; Andersen, C. Yding. / Germ cell numbers in human embryonic and fetal gonads during the first two trimesters of pregnancy : Analysis of six published studies. In: Human Reproduction. 2011 ; Vol. 26, No. 8. pp. 2140-2145.
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title = "Germ cell numbers in human embryonic and fetal gonads during the first two trimesters of pregnancy: Analysis of six published studies",
abstract = "BACKGROUND: The number of germ cells in human embryonic and fetal ovaries in relation to age is currently based on volumetric estimations from one study including a total of 12 ovaries. Six recent publications present stereological estimations of the number of germ cells in ovaries and testes for the first two trimesters. ; METHODS: Germ cell numbers from 103 human first and second trimester gonads aged 37–133 days post-conception (p.c.), obtained after legal termination of pregnancy, were collected from six independent studies that all used similar validated stereological methods for estimating germ cell numbers as well as somatic cell numbers. ; RESULTS: Statistically, the six studies estimated similar number of germ cells (P > 0.05) and no interaction between the studies and age was found (P > 0.05), indicating that the increase in cell numbers in relation to age was of comparable magnitude in each study. The number of germ cells increased from a mean of 7200 to 4 933 000 in fetal ovaries and from 3700 to 1 417 000 in fetal testes, from week 5 to week 19 p.c. A higher rate of increase was found for female germ cells as compared with males (P = 0.004). During the same period, the number of somatic cells increased from a mean of 158 000 to 1 017 000 in ovaries and from 154 000 to 2 035 000 in testes, respectively. ; CONCLUSIONS: By the use of validated stereological methods, this study provides more accurate and improved information on human germ and somatic cell numbers in ovaries and testes during the first two trimesters of pregnancy.",
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author = "Mamsen, {L. Salto} and M.C. Lutterodt and Andersen, {Elisabeth Wreford} and A.G. Byskov and Andersen, {C. Yding}",
year = "2011",
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Germ cell numbers in human embryonic and fetal gonads during the first two trimesters of pregnancy : Analysis of six published studies. / Mamsen, L. Salto; Lutterodt, M.C.; Andersen, Elisabeth Wreford; Byskov, A.G.; Andersen, C. Yding.

In: Human Reproduction, Vol. 26, No. 8, 2011, p. 2140-2145.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Germ cell numbers in human embryonic and fetal gonads during the first two trimesters of pregnancy

T2 - Analysis of six published studies

AU - Mamsen, L. Salto

AU - Lutterodt, M.C.

AU - Andersen, Elisabeth Wreford

AU - Byskov, A.G.

AU - Andersen, C. Yding

PY - 2011

Y1 - 2011

N2 - BACKGROUND: The number of germ cells in human embryonic and fetal ovaries in relation to age is currently based on volumetric estimations from one study including a total of 12 ovaries. Six recent publications present stereological estimations of the number of germ cells in ovaries and testes for the first two trimesters. ; METHODS: Germ cell numbers from 103 human first and second trimester gonads aged 37–133 days post-conception (p.c.), obtained after legal termination of pregnancy, were collected from six independent studies that all used similar validated stereological methods for estimating germ cell numbers as well as somatic cell numbers. ; RESULTS: Statistically, the six studies estimated similar number of germ cells (P > 0.05) and no interaction between the studies and age was found (P > 0.05), indicating that the increase in cell numbers in relation to age was of comparable magnitude in each study. The number of germ cells increased from a mean of 7200 to 4 933 000 in fetal ovaries and from 3700 to 1 417 000 in fetal testes, from week 5 to week 19 p.c. A higher rate of increase was found for female germ cells as compared with males (P = 0.004). During the same period, the number of somatic cells increased from a mean of 158 000 to 1 017 000 in ovaries and from 154 000 to 2 035 000 in testes, respectively. ; CONCLUSIONS: By the use of validated stereological methods, this study provides more accurate and improved information on human germ and somatic cell numbers in ovaries and testes during the first two trimesters of pregnancy.

AB - BACKGROUND: The number of germ cells in human embryonic and fetal ovaries in relation to age is currently based on volumetric estimations from one study including a total of 12 ovaries. Six recent publications present stereological estimations of the number of germ cells in ovaries and testes for the first two trimesters. ; METHODS: Germ cell numbers from 103 human first and second trimester gonads aged 37–133 days post-conception (p.c.), obtained after legal termination of pregnancy, were collected from six independent studies that all used similar validated stereological methods for estimating germ cell numbers as well as somatic cell numbers. ; RESULTS: Statistically, the six studies estimated similar number of germ cells (P > 0.05) and no interaction between the studies and age was found (P > 0.05), indicating that the increase in cell numbers in relation to age was of comparable magnitude in each study. The number of germ cells increased from a mean of 7200 to 4 933 000 in fetal ovaries and from 3700 to 1 417 000 in fetal testes, from week 5 to week 19 p.c. A higher rate of increase was found for female germ cells as compared with males (P = 0.004). During the same period, the number of somatic cells increased from a mean of 158 000 to 1 017 000 in ovaries and from 154 000 to 2 035 000 in testes, respectively. ; CONCLUSIONS: By the use of validated stereological methods, this study provides more accurate and improved information on human germ and somatic cell numbers in ovaries and testes during the first two trimesters of pregnancy.

KW - Germ cells

KW - Human embryos

KW - First and second trimester pregnancy

U2 - 10.1093/humrep/der149

DO - 10.1093/humrep/der149

M3 - Journal article

VL - 26

SP - 2140

EP - 2145

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 8

ER -