TY - JOUR
T1 - Gentamicin susceptibility in Escherichia coli related to the genetic background: problems with breakpoints
AU - Jakobsen, L.
AU - Sandvang, D.
AU - Jensen, Vibeke Frøkjær
AU - Seyfarth, Anne Mette
AU - Frimødt-Møller, N.
AU - Hammerum, Anette Marie
PY - 2007
Y1 - 2007
N2 - In total, 120 Escherichia coli isolates positive for one of the gentamicin resistance (GEN(R)) genes aac(3)-II, aac(3)-IV or ant(2 '')-I were tested for gentamicin susceptibility by the agar dilution method. Isolates positive for aac(3)-IV or ant(2 '')-I had an MIC distribution of 8-64 mg/L, whereas isolates positive for aac(3)-II had MICs of 32 to > 512 mg/L, suggesting a relationship between the distribution of MICs and the specific GEN(R) mechanism. The MIC distribution, regardless of the GEN(R) mechanism, was 8 - > 512 mg/L, which supports the clinical breakpoint of MIC > 4 mg/L suggested by EUCAST and questions the breakpoint recommended by the CLSI (>= 16 mg/L).
AB - In total, 120 Escherichia coli isolates positive for one of the gentamicin resistance (GEN(R)) genes aac(3)-II, aac(3)-IV or ant(2 '')-I were tested for gentamicin susceptibility by the agar dilution method. Isolates positive for aac(3)-IV or ant(2 '')-I had an MIC distribution of 8-64 mg/L, whereas isolates positive for aac(3)-II had MICs of 32 to > 512 mg/L, suggesting a relationship between the distribution of MICs and the specific GEN(R) mechanism. The MIC distribution, regardless of the GEN(R) mechanism, was 8 - > 512 mg/L, which supports the clinical breakpoint of MIC > 4 mg/L suggested by EUCAST and questions the breakpoint recommended by the CLSI (>= 16 mg/L).
KW - gentamicin
KW - susceptibility tests
KW - resistance genes
KW - MIC distribution
KW - Escherichia coli
KW - breakpoints
U2 - 10.1111/j.1469-0691.2007.01751.x
DO - 10.1111/j.1469-0691.2007.01751.x
M3 - Journal article
SN - 1198-743X
VL - 13
SP - 830
EP - 832
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
IS - 8
ER -