Genotyping of human and porcine Yersinia enterocolitica, Yersinia intertmedia, and Yersinia bercovieri strains from Switzerland by amplified fragment length polymorphism analysis

Kathrin Kuehni-Boghenbor, Stephen L.W. On, Branko Kokotovic, Andreas Baumgartner, Trudy M. Wassenaar, Matthias Wittwer, Beatrice Bissig-Choisat, Joachim Frey

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In this study, 231 strains of Yersinia enterocolitica, 25 strains of Y. intermedia, and 10 strains of Y. bercovieri from human and porcine sources (including reference strains) were analyzed using amplified fragment length polymorphism (AFLP), a whole-genome fingerprinting method for subtyping bacterial isolates. AFLP typing distinguished the different Yersinia species examined. Representatives of Y. enterocolitica biotypes 1A, 1B, 2, 3, and 4 belonged to biotype-related AFLP clusters and were clearly distinguished from each other. Y. enterocolitica biotypes 2, 3, and 4 appeared to be more closely related to each other (83% similarity) than to biotypes 1A (11%) and 1B (47%). Biotype 1A strains exhibited the greatest genetic heterogeneity of the biotypes studied. The biotype 1A genotypes were distributed among four major clusters, each containing strains from both human and porcine sources, confirming the zoonotic potential of this organism. The AFLP technique is a valuable genotypic method for identification and typing of Y. enterocolitica and other Yersinia spp.
    Original languageEnglish
    JournalApplied and Environmental Microbiology
    Volume72
    Issue number6
    Pages (from-to)4061-4066
    ISSN0099-2240
    DOIs
    Publication statusPublished - 2006

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