TY - JOUR
T1 - Genotyping and antimicrobial susceptibility profiling of streptococcus uberis isolated from a clinical bovine mastitis outbreak in a dairy farm
AU - Monistero, Valentina
AU - Barberio, Antonio
AU - Cremonesi, Paola
AU - Castiglioni, Bianca
AU - Morandi, Stefano
AU - Lassen, Desiree C.K.
AU - Astrup, Lærke B.
AU - Locatelli, Clara
AU - Piccinini, Renata
AU - Filippa Addis, M.
AU - Bronzo, Valerio
AU - Moroni, Paolo
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - Streptococcus uberis, an environmental pathogen responsible also for contagious transmis-sion, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of Strep. uberis CM cases in an Italian dairy farm. We deter-mined molecular characteristics and phenotypic antimicrobial resistance of 71 Strep. uberis isolates from dairy cows with CM. Genotypic variability was investigated via multiplex PCR of housekeeping and virulence genes, and by RAPD-PCR typing. Antimicrobial susceptibility was assessed for 14 antimicrobials by MIC assay. All the isolates carried the 11 genes investigated. At 90% similarity, two distinct clusters, grouping 69 of the 71 isolates, were detected in the dendrogram derived from the primer ERIC1. The predominant cluster I could be separated into two subclusters, containing 38 and 14 isolates, respectively. Strep. uberis strains belonging to the same RAPD pattern differed in their resistance profiles. Most (97.2%) of them were resistant to at least one of the drugs tested, but only 25.4% showed a multidrug resistance phenotype. The highest resistance rate was observed for lincomycin (93%), followed by tetracycline (85.9%). This study confirmed a low prevalence of β-lactam resistance in Strep. uberis, with only one isolate showing resistance to six antimicrobial classes, including cephalosporins.
AB - Streptococcus uberis, an environmental pathogen responsible also for contagious transmis-sion, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of Strep. uberis CM cases in an Italian dairy farm. We deter-mined molecular characteristics and phenotypic antimicrobial resistance of 71 Strep. uberis isolates from dairy cows with CM. Genotypic variability was investigated via multiplex PCR of housekeeping and virulence genes, and by RAPD-PCR typing. Antimicrobial susceptibility was assessed for 14 antimicrobials by MIC assay. All the isolates carried the 11 genes investigated. At 90% similarity, two distinct clusters, grouping 69 of the 71 isolates, were detected in the dendrogram derived from the primer ERIC1. The predominant cluster I could be separated into two subclusters, containing 38 and 14 isolates, respectively. Strep. uberis strains belonging to the same RAPD pattern differed in their resistance profiles. Most (97.2%) of them were resistant to at least one of the drugs tested, but only 25.4% showed a multidrug resistance phenotype. The highest resistance rate was observed for lincomycin (93%), followed by tetracycline (85.9%). This study confirmed a low prevalence of β-lactam resistance in Strep. uberis, with only one isolate showing resistance to six antimicrobial classes, including cephalosporins.
KW - Clinical mastitis
KW - Cow
KW - MIC
KW - RAPD
KW - Streptococcus uberis
U2 - 10.3390/antibiotics10060644
DO - 10.3390/antibiotics10060644
M3 - Journal article
C2 - 34071296
AN - SCOPUS:85107851755
SN - 2079-6382
VL - 10
JO - Antibiotics
JF - Antibiotics
IS - 6
M1 - 644
ER -