Genotoxicity of clays with potential use in biopolymers for food packaging

Anoop Kumar Sharma, Alicja Mortensen, Niels Hadrup, Vivian Jørgensen, Mona-Lise Binderup

Research output: Contribution to conferencePosterResearch

Abstract

Genotoxicity of clays with potential use in biopolymers for food packaging Plastics produced from biopolymers are of commercial interest as they are manufactured from renewable resources such as agricultural crop wastes and have the potential to meet environmental and health requirements. Biopolymers that are strengthened using reinforcing nano-scale fillers may improve the packaging quality by increasing barrier function and heat-resistance. Toxicological data on clays containing a nano-fraction and organo-modified clays remain very limited. The aim of this study is to investigate the genotoxic potential of clays that can be used in biopolymers for food contact materials. Two clays were tested in the comet assay using Caco-2 cells (a human colon cancer cell line); a natural montmorillonite (Cloisite®Na+) and an organo-modified montmorillonite (Cloisite®30B). Both clays were tested in crude suspensions (suspended in cell culture medium) and crude suspensions filtrated through a 0.2 µm pore size filter in order to investigate the potential effect of “nanoparticles” only. The two clays showed noticeable differences in genotoxicity; both crude and filtered suspensions of Cloisite®Na+ showed negative results, while crude and filtered suspensions of Cloisite®30B were genotoxic in a clear concentration related manner. Analysis of inorganic elements and particle size distributions of filtered suspensions of Cloisite®30B indicated that no clay particle were present. However, the organo-modifier was detected in filtered suspensions of Cloisite®30B by HPLC-MS, thus indicating that the organo-modifier was at least partly responsible for the genotoxic effect. As a follow up on the in vitro results the compounds will be tested in an in vivo comet assay experiment. Wistar rats will be exposed to Cloisite®30B and Cloisite®Na+ by oral gavage and the comet assay will be performed on cells from different organs including the liver, colon and kidney. A fully automated comet assay scoring system (Imstar) will be used to evaluate the genotoxic potential.
Original languageEnglish
Publication date2010
Publication statusPublished - 2010
Event12th International Congress of Toxicology - Barcelona, Spain
Duration: 19 Jul 201023 Jul 2010
Conference number: 12

Conference

Conference12th International Congress of Toxicology
Number12
CountrySpain
CityBarcelona
Period19/07/201023/07/2010

Keywords

  • Toxicology

Cite this

Sharma, A. K., Mortensen, A., Hadrup, N., Jørgensen, V., & Binderup, M-L. (2010). Genotoxicity of clays with potential use in biopolymers for food packaging. Poster session presented at 12th International Congress of Toxicology, Barcelona, Spain.
Sharma, Anoop Kumar ; Mortensen, Alicja ; Hadrup, Niels ; Jørgensen, Vivian ; Binderup, Mona-Lise. / Genotoxicity of clays with potential use in biopolymers for food packaging. Poster session presented at 12th International Congress of Toxicology, Barcelona, Spain.
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abstract = "Genotoxicity of clays with potential use in biopolymers for food packaging Plastics produced from biopolymers are of commercial interest as they are manufactured from renewable resources such as agricultural crop wastes and have the potential to meet environmental and health requirements. Biopolymers that are strengthened using reinforcing nano-scale fillers may improve the packaging quality by increasing barrier function and heat-resistance. Toxicological data on clays containing a nano-fraction and organo-modified clays remain very limited. The aim of this study is to investigate the genotoxic potential of clays that can be used in biopolymers for food contact materials. Two clays were tested in the comet assay using Caco-2 cells (a human colon cancer cell line); a natural montmorillonite (Cloisite{\circledR}Na+) and an organo-modified montmorillonite (Cloisite{\circledR}30B). Both clays were tested in crude suspensions (suspended in cell culture medium) and crude suspensions filtrated through a 0.2 µm pore size filter in order to investigate the potential effect of “nanoparticles” only. The two clays showed noticeable differences in genotoxicity; both crude and filtered suspensions of Cloisite{\circledR}Na+ showed negative results, while crude and filtered suspensions of Cloisite{\circledR}30B were genotoxic in a clear concentration related manner. Analysis of inorganic elements and particle size distributions of filtered suspensions of Cloisite{\circledR}30B indicated that no clay particle were present. However, the organo-modifier was detected in filtered suspensions of Cloisite{\circledR}30B by HPLC-MS, thus indicating that the organo-modifier was at least partly responsible for the genotoxic effect. As a follow up on the in vitro results the compounds will be tested in an in vivo comet assay experiment. Wistar rats will be exposed to Cloisite{\circledR}30B and Cloisite{\circledR}Na+ by oral gavage and the comet assay will be performed on cells from different organs including the liver, colon and kidney. A fully automated comet assay scoring system (Imstar) will be used to evaluate the genotoxic potential.",
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author = "Sharma, {Anoop Kumar} and Alicja Mortensen and Niels Hadrup and Vivian J{\o}rgensen and Mona-Lise Binderup",
year = "2010",
language = "English",
note = "12th International Congress of Toxicology, IUTOX 2010 ; Conference date: 19-07-2010 Through 23-07-2010",

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Sharma, AK, Mortensen, A, Hadrup, N, Jørgensen, V & Binderup, M-L 2010, 'Genotoxicity of clays with potential use in biopolymers for food packaging', 12th International Congress of Toxicology, Barcelona, Spain, 19/07/2010 - 23/07/2010.

Genotoxicity of clays with potential use in biopolymers for food packaging. / Sharma, Anoop Kumar; Mortensen, Alicja; Hadrup, Niels; Jørgensen, Vivian; Binderup, Mona-Lise.

2010. Poster session presented at 12th International Congress of Toxicology, Barcelona, Spain.

Research output: Contribution to conferencePosterResearch

TY - CONF

T1 - Genotoxicity of clays with potential use in biopolymers for food packaging

AU - Sharma, Anoop Kumar

AU - Mortensen, Alicja

AU - Hadrup, Niels

AU - Jørgensen, Vivian

AU - Binderup, Mona-Lise

PY - 2010

Y1 - 2010

N2 - Genotoxicity of clays with potential use in biopolymers for food packaging Plastics produced from biopolymers are of commercial interest as they are manufactured from renewable resources such as agricultural crop wastes and have the potential to meet environmental and health requirements. Biopolymers that are strengthened using reinforcing nano-scale fillers may improve the packaging quality by increasing barrier function and heat-resistance. Toxicological data on clays containing a nano-fraction and organo-modified clays remain very limited. The aim of this study is to investigate the genotoxic potential of clays that can be used in biopolymers for food contact materials. Two clays were tested in the comet assay using Caco-2 cells (a human colon cancer cell line); a natural montmorillonite (Cloisite®Na+) and an organo-modified montmorillonite (Cloisite®30B). Both clays were tested in crude suspensions (suspended in cell culture medium) and crude suspensions filtrated through a 0.2 µm pore size filter in order to investigate the potential effect of “nanoparticles” only. The two clays showed noticeable differences in genotoxicity; both crude and filtered suspensions of Cloisite®Na+ showed negative results, while crude and filtered suspensions of Cloisite®30B were genotoxic in a clear concentration related manner. Analysis of inorganic elements and particle size distributions of filtered suspensions of Cloisite®30B indicated that no clay particle were present. However, the organo-modifier was detected in filtered suspensions of Cloisite®30B by HPLC-MS, thus indicating that the organo-modifier was at least partly responsible for the genotoxic effect. As a follow up on the in vitro results the compounds will be tested in an in vivo comet assay experiment. Wistar rats will be exposed to Cloisite®30B and Cloisite®Na+ by oral gavage and the comet assay will be performed on cells from different organs including the liver, colon and kidney. A fully automated comet assay scoring system (Imstar) will be used to evaluate the genotoxic potential.

AB - Genotoxicity of clays with potential use in biopolymers for food packaging Plastics produced from biopolymers are of commercial interest as they are manufactured from renewable resources such as agricultural crop wastes and have the potential to meet environmental and health requirements. Biopolymers that are strengthened using reinforcing nano-scale fillers may improve the packaging quality by increasing barrier function and heat-resistance. Toxicological data on clays containing a nano-fraction and organo-modified clays remain very limited. The aim of this study is to investigate the genotoxic potential of clays that can be used in biopolymers for food contact materials. Two clays were tested in the comet assay using Caco-2 cells (a human colon cancer cell line); a natural montmorillonite (Cloisite®Na+) and an organo-modified montmorillonite (Cloisite®30B). Both clays were tested in crude suspensions (suspended in cell culture medium) and crude suspensions filtrated through a 0.2 µm pore size filter in order to investigate the potential effect of “nanoparticles” only. The two clays showed noticeable differences in genotoxicity; both crude and filtered suspensions of Cloisite®Na+ showed negative results, while crude and filtered suspensions of Cloisite®30B were genotoxic in a clear concentration related manner. Analysis of inorganic elements and particle size distributions of filtered suspensions of Cloisite®30B indicated that no clay particle were present. However, the organo-modifier was detected in filtered suspensions of Cloisite®30B by HPLC-MS, thus indicating that the organo-modifier was at least partly responsible for the genotoxic effect. As a follow up on the in vitro results the compounds will be tested in an in vivo comet assay experiment. Wistar rats will be exposed to Cloisite®30B and Cloisite®Na+ by oral gavage and the comet assay will be performed on cells from different organs including the liver, colon and kidney. A fully automated comet assay scoring system (Imstar) will be used to evaluate the genotoxic potential.

KW - Toxicology

M3 - Poster

ER -

Sharma AK, Mortensen A, Hadrup N, Jørgensen V, Binderup M-L. Genotoxicity of clays with potential use in biopolymers for food packaging. 2010. Poster session presented at 12th International Congress of Toxicology, Barcelona, Spain.