Genomic variations of Mycoplasma capricolum subsp capripneumoniae detected by amplified fragment length polymorphism (AFLP) analysis

Branko Kokotovic, G. Bolske, Peter Ahrens, K.E. Johansson

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The genetic diversity of Mycoplasma capricolum subsp. capripneumoniae strains based on determination of amplified fragment length polymorphisms (AFLP) is described. AFLP fingerprints of 38 strains derived from different countries in Africa and the Middle East consisted of over 100 bands in the size range of 40-500 bp. The similarity between individual AFLP profiles, calculated by Jaccard's coefficient, ranged from 0.92 to 1.0. On the basis of the polymorphisms detected, the analysed strains can explicitly be grouped into two major clusters, equivalent to two evolutionary lines of the organism found by 16S rDNA analysis. The present data support previous observations regarding genetic homogeneity of M. capricolum subsp. capripneumoniae, and confirm the two evolutionary lines of descent found by analysis of 16S rRNA genes.
    Original languageEnglish
    JournalFEMS Microbiology Letters
    Volume184
    Issue number1
    Pages (from-to)63-68
    ISSN0378-1097
    Publication statusPublished - 2000

    Keywords

    • Mollicutes
    • Mycoplasma capricolum subsp capripneumoniae
    • genomic fingerprinting
    • contagious caprine pleuropneumonia
    • amplified fragment length polymorphism
    • molecular typing

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