Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis

V. Fussing

doi:10.1046/j.1472-765X.1998.t01-1-00419.x

Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis

V. Fussing

Technical University of Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The aim of the present study was to examine the genomic relationship among 112 Actinobacillus pleuropneumoniae serotype 2 strains obtained throughout Europe and North America. HindIII ribotyping of the strains resulted in five ribotypes of high similarity (87-98%). Sequence analysis of the ribosomal intergenic region of strains representing each ribotype and each country showed no differences. A common ribotype was further characterized by PFGE of 12 strains representing all countries. The resultant five PFGE patterns of European strains showed a similarity of more than 91%, to which the two PFGE patterns of the Canadian strains showed a similarity of 84-86%. In conclusion, strains of A, pleuropneumoniae serotype 2 seem to be clonally related.

Original language	English
Journal	Letters in Applied Microbiology
Volume	27
Issue number	4
Pages (from-to)	211-215
ISSN	0266-8254
DOIs	https://doi.org/10.1046/j.1472-765X.1998.t01-1-00419.x
Publication status	Published - 1998

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title = "Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis",

abstract = "The aim of the present study was to examine the genomic relationship among 112 Actinobacillus pleuropneumoniae serotype 2 strains obtained throughout Europe and North America. HindIII ribotyping of the strains resulted in five ribotypes of high similarity (87-98\%). Sequence analysis of the ribosomal intergenic region of strains representing each ribotype and each country showed no differences. A common ribotype was further characterized by PFGE of 12 strains representing all countries. The resultant five PFGE patterns of European strains showed a similarity of more than 91\%, to which the two PFGE patterns of the Canadian strains showed a similarity of 84-86\%. In conclusion, strains of A, pleuropneumoniae serotype 2 seem to be clonally related.",

author = "V. Fussing",

year = "1998",

doi = "10.1046/j.1472-765X.1998.t01-1-00419.x",

language = "English",

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journal = "Letters in Applied Microbiology",

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TY - JOUR

T1 - Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis

AU - Fussing, V.

PY - 1998

Y1 - 1998

N2 - The aim of the present study was to examine the genomic relationship among 112 Actinobacillus pleuropneumoniae serotype 2 strains obtained throughout Europe and North America. HindIII ribotyping of the strains resulted in five ribotypes of high similarity (87-98%). Sequence analysis of the ribosomal intergenic region of strains representing each ribotype and each country showed no differences. A common ribotype was further characterized by PFGE of 12 strains representing all countries. The resultant five PFGE patterns of European strains showed a similarity of more than 91%, to which the two PFGE patterns of the Canadian strains showed a similarity of 84-86%. In conclusion, strains of A, pleuropneumoniae serotype 2 seem to be clonally related.

AB - The aim of the present study was to examine the genomic relationship among 112 Actinobacillus pleuropneumoniae serotype 2 strains obtained throughout Europe and North America. HindIII ribotyping of the strains resulted in five ribotypes of high similarity (87-98%). Sequence analysis of the ribosomal intergenic region of strains representing each ribotype and each country showed no differences. A common ribotype was further characterized by PFGE of 12 strains representing all countries. The resultant five PFGE patterns of European strains showed a similarity of more than 91%, to which the two PFGE patterns of the Canadian strains showed a similarity of 84-86%. In conclusion, strains of A, pleuropneumoniae serotype 2 seem to be clonally related.

U2 - 10.1046/j.1472-765X.1998.t01-1-00419.x

DO - 10.1046/j.1472-765X.1998.t01-1-00419.x

M3 - Journal article

SN - 0266-8254

VL - 27

SP - 211

EP - 215

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 4

ER -

Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis

Abstract

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