Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis

V. Fussing

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    Abstract

    The aim of the present study was to examine the genomic relationship among 112 Actinobacillus pleuropneumoniae serotype 2 strains obtained throughout Europe and North America. HindIII ribotyping of the strains resulted in five ribotypes of high similarity (87-98%). Sequence analysis of the ribosomal intergenic region of strains representing each ribotype and each country showed no differences. A common ribotype was further characterized by PFGE of 12 strains representing all countries. The resultant five PFGE patterns of European strains showed a similarity of more than 91%, to which the two PFGE patterns of the Canadian strains showed a similarity of 84-86%. In conclusion, strains of A, pleuropneumoniae serotype 2 seem to be clonally related.
    Original languageEnglish
    JournalLetters in Applied Microbiology
    Volume27
    Issue number4
    Pages (from-to)211-215
    ISSN0266-8254
    DOIs
    Publication statusPublished - 1998

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