Genomic insights into the glutathione metabolism of the wine yeast Starmerella bacillaris

Wilson José Fernandes Lemos Junior, Laura Treu, Chiara Nadai, Vinícius da Silva Duarte, Stefano Campanaro, Marta Fabrega-Prats, Alessio Giacomini*, Viviana Corich

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Glutathione (GSH) is an antioxidant molecule of great technological interest due to its wide range of applications in the food and beverage industry. In winemaking, although glutathione is produced during fermentation, its addition is possible for the control of oxidative spoilage of wine. Recently, to improve wine quality, mixed fermentation has been proposed by introducing a selection of non-Saccharomyces yeasts as complementary starters to the oenological species S. cerevisiae. Among them, Starmerella bacillaris, an osmophilic and high glycerol producer yeast, has been extensively studied. In the present study, the genomes of two S. bacillaris strains were compared with S. cerevisiae to identify the GSH metabolic pathway. The results showed that GSH biosynthesis includes the GSH1 and GSH2 genes in both species. The identification of a new transcription factor which binds sites in the promoter region of these genes underlined differences in the transcriptional regulation of both species. Additionally, between S. bacillaris strains, a high number of polymorphisms was found in genes involved in GSH redox balance. Preliminary laboratory scale fermentations revealed marked differences in the cell glutathione content of the two S. bacillaris strains. By comparing genomes, it was possible to gain a better understanding of the genes involved in the GSH metabolism pathway in S. bacillaris.

Original languageEnglish
JournalOeno One
Volume55
Issue number1
Pages (from-to)105-117
ISSN2494-1271
DOIs
Publication statusPublished - 2021

Bibliographical note

Publisher Copyright:
© 2021 Vigne et Vin Publications Internationales. All rights reserved.

Keywords

  • Candida zemplinina
  • Glutathione
  • GSH1
  • GSH2
  • Saccharomyces cerevisiae

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