Genomic Evolution Of The Mdr Serotype O12 Pseudomonas Aeruginosa Clone

Sandra Wingaard Thrane, Véronique L. Taylor, Luca Freschi, Irena Kukavica-Ibrulj, Brian Boyle, Jérôme Laroche, Jean-Paul Pirnay, Roger C. Lévesque, Joseph S. Lam, Lars Jelsbak

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    Abstract

    Introduction: Since the 1980’s the serotype O12 of Pseudomonas aeruginosa has emerged as the predominant serotype in clinical settings and in epidemic outbreaks. These serotype O12 isolates exhibit high levels of resistance to various classes of antibiotics.Methods: In this study, we explore how the P. aeruginosa LPS biosynthesis gene clusters evolve in the population by investigating the phylogenetic relationship among 83 P. aeruginosa strains and their serotype. In the process we develop a program for in silico serotyping of P. aeruginosa isolates, the P. aeruginosa serotyper (PAst).Results: While most serotypes were closely linked to the core genome phylogeny we observed horizontal exchange of LPS genes among distinct P. aeruginosa strains. Specifically, we identified a ‘serotype island’ containing the P. aeruginosa O12 LPS gene cluster and an antibiotic resistance determinant (gyrAC248T) that has been transferred among P. aeruginosa strains. Acquisition and recombination of the ‘serotype island’ resulted in expression of the O12 serotype in the recipient strains. Conclusions: This observation demonstrate a strong selective advantage for this type of genomic recombination, and suggest that serotype switching in combination with an antibiotic resistance determinant contributed to the dissemination of the O12 serotype in the clinic. This selective advantage coincides with the introduction of fluoroquinolones in the clinic. With the PAst program isolates can be serotyped using WGS data, and dangerous clones like O12 can be identified quickly.
    Original languageEnglish
    Title of host publicationThe Danish Microbiological Society Annual Congress 2015 : Programme & Abstracts
    Place of PublicationCopenhagen
    Publication date2015
    Pages48-48
    Publication statusPublished - 2015
    EventThe Danish Microbiological Society Annual Congress 2015 - Eigtved's Pakhus, Copenhagen, Denmark
    Duration: 9 Nov 20159 Nov 2015

    Conference

    ConferenceThe Danish Microbiological Society Annual Congress 2015
    LocationEigtved's Pakhus
    CountryDenmark
    CityCopenhagen
    Period09/11/201509/11/2015

    Cite this

    Thrane, S. W., Taylor, V. L., Freschi, L., Kukavica-Ibrulj, I., Boyle, B., Laroche, J., Pirnay, J-P., Lévesque, R. C., Lam, J. S., & Jelsbak, L. (2015). Genomic Evolution Of The Mdr Serotype O12 Pseudomonas Aeruginosa Clone. In The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts (pp. 48-48).