Genomic epidemiology of a major Mycobacterium tuberculosis outbreak: Retrospective cohort study in a low incidence setting using sparse time-series sampling

Dorte Bek Folkvardsen, Anders Norman, Åse Bengård Andersen, Erik Michael Rasmussen, Lars Jelsbak, Troels Lillebaek

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Abstract

Since 1992, Denmark has documented the largest outbreak of tuberculosis in Scandinavia ascribed to a single genotype, termed 'C2/1112-15'. As of spring 2017, the International Reference Laboratory of Mycobacteriology in Copenhagen has collected and identified isolates from more than a thousand cases belonging to this outbreak via routine MIRU-VNTR typing. Here, we present a retrospective analysis of the C2/1112-15 dataset, based on whole-genome data from a sparse time-series consisting of five randomly selected isolates from each of the 23 years. Even if these data are derived from only 12% of the collected isolates, we have been able to extract important key information, such as mutation rate, conserved single-nucleotide polymorphisms to identify discrete transmission chains, as well as the possible historical origins of the outbreak.
Original languageEnglish
JournalJournal of Infectious Diseases
Volume216
Issue number3
Number of pages19
ISSN0022-1899
DOIs
Publication statusPublished - 2017

Cite this

Folkvardsen, Dorte Bek ; Norman, Anders ; Andersen, Åse Bengård ; Rasmussen, Erik Michael ; Jelsbak, Lars ; Lillebaek, Troels. / Genomic epidemiology of a major Mycobacterium tuberculosis outbreak: Retrospective cohort study in a low incidence setting using sparse time-series sampling. In: Journal of Infectious Diseases. 2017 ; Vol. 216, No. 3.
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Genomic epidemiology of a major Mycobacterium tuberculosis outbreak: Retrospective cohort study in a low incidence setting using sparse time-series sampling. / Folkvardsen, Dorte Bek; Norman, Anders; Andersen, Åse Bengård; Rasmussen, Erik Michael; Jelsbak, Lars; Lillebaek, Troels.

In: Journal of Infectious Diseases, Vol. 216, No. 3, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Jelsbak, Lars

AU - Lillebaek, Troels

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AB - Since 1992, Denmark has documented the largest outbreak of tuberculosis in Scandinavia ascribed to a single genotype, termed 'C2/1112-15'. As of spring 2017, the International Reference Laboratory of Mycobacteriology in Copenhagen has collected and identified isolates from more than a thousand cases belonging to this outbreak via routine MIRU-VNTR typing. Here, we present a retrospective analysis of the C2/1112-15 dataset, based on whole-genome data from a sparse time-series consisting of five randomly selected isolates from each of the 23 years. Even if these data are derived from only 12% of the collected isolates, we have been able to extract important key information, such as mutation rate, conserved single-nucleotide polymorphisms to identify discrete transmission chains, as well as the possible historical origins of the outbreak.

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