Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme

Tatiana Rochat, Erina Fujiwara-Nagata, Ségolène Calvez, Inger Dalsgaard, Lone Madsen, Alexandra Calteau, Aurélie Lunazzi, Pierre Nicolas, Tom Wiklund, Jean-Francois Bernardet, Eric Duchaud

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    Abstract

    Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.
    Original languageEnglish
    Article number1752
    JournalFrontiers in Microbiology
    Volume8
    ISSN1664-302X
    DOIs
    Publication statusPublished - 2017

    Bibliographical note

    This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

    Keywords

    • mPCR
    • Fish-pathogenic bacteria
    • Flavobacterium psychrophilum
    • Genomics
    • Salmonid aquaculture
    • Serotype

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