Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme

Tatiana Rochat, Erina Fujiwara-Nagata, Ségolène Calvez, Inger Dalsgaard, Lone Madsen, Alexandra Calteau, Aurélie Lunazzi, Pierre Nicolas, Tom Wiklund, Jean-Francois Bernardet, Eric Duchaud

    Research output: Contribution to journalJournal articleResearchpeer-review

    238 Downloads (Pure)

    Abstract

    Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.
    Original languageEnglish
    Article number1752
    JournalFrontiers in Microbiology
    Volume8
    ISSN1664-302X
    DOIs
    Publication statusPublished - 2017

    Bibliographical note

    This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

    Keywords

    • mPCR
    • Fish-pathogenic bacteria
    • Flavobacterium psychrophilum
    • Genomics
    • Salmonid aquaculture
    • Serotype

    Cite this

    Rochat, Tatiana ; Fujiwara-Nagata, Erina ; Calvez, Ségolène ; Dalsgaard, Inger ; Madsen, Lone ; Calteau, Alexandra ; Lunazzi, Aurélie ; Nicolas, Pierre ; Wiklund, Tom ; Bernardet, Jean-Francois ; Duchaud, Eric. / Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme. In: Frontiers in Microbiology. 2017 ; Vol. 8.
    @article{ba2a55e6b0884e0a8fe205535cdf9671,
    title = "Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme",
    abstract = "Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.",
    keywords = "mPCR, Fish-pathogenic bacteria, Flavobacterium psychrophilum, Genomics, Salmonid aquaculture, Serotype",
    author = "Tatiana Rochat and Erina Fujiwara-Nagata and S{\'e}gol{\`e}ne Calvez and Inger Dalsgaard and Lone Madsen and Alexandra Calteau and Aur{\'e}lie Lunazzi and Pierre Nicolas and Tom Wiklund and Jean-Francois Bernardet and Eric Duchaud",
    note = "This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).",
    year = "2017",
    doi = "10.3389/fmicb.2017.01752",
    language = "English",
    volume = "8",
    journal = "Frontiers in Microbiology",
    issn = "1664-302X",
    publisher = "Frontiers Media S.A.",

    }

    Rochat, T, Fujiwara-Nagata, E, Calvez, S, Dalsgaard, I, Madsen, L, Calteau, A, Lunazzi, A, Nicolas, P, Wiklund, T, Bernardet, J-F & Duchaud, E 2017, 'Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme', Frontiers in Microbiology, vol. 8, 1752. https://doi.org/10.3389/fmicb.2017.01752

    Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme. / Rochat, Tatiana; Fujiwara-Nagata, Erina; Calvez, Ségolène; Dalsgaard, Inger; Madsen, Lone; Calteau, Alexandra; Lunazzi, Aurélie; Nicolas, Pierre; Wiklund, Tom; Bernardet, Jean-Francois; Duchaud, Eric.

    In: Frontiers in Microbiology, Vol. 8, 1752, 2017.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme

    AU - Rochat, Tatiana

    AU - Fujiwara-Nagata, Erina

    AU - Calvez, Ségolène

    AU - Dalsgaard, Inger

    AU - Madsen, Lone

    AU - Calteau, Alexandra

    AU - Lunazzi, Aurélie

    AU - Nicolas, Pierre

    AU - Wiklund, Tom

    AU - Bernardet, Jean-Francois

    AU - Duchaud, Eric

    N1 - This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

    PY - 2017

    Y1 - 2017

    N2 - Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.

    AB - Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.

    KW - mPCR

    KW - Fish-pathogenic bacteria

    KW - Flavobacterium psychrophilum

    KW - Genomics

    KW - Salmonid aquaculture

    KW - Serotype

    U2 - 10.3389/fmicb.2017.01752

    DO - 10.3389/fmicb.2017.01752

    M3 - Journal article

    VL - 8

    JO - Frontiers in Microbiology

    JF - Frontiers in Microbiology

    SN - 1664-302X

    M1 - 1752

    ER -