Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme

Tatiana Rochat; Erina Fujiwara-Nagata; Ségolène Calvez; Inger Dalsgaard; Lone Madsen; Alexandra Calteau; Aurélie Lunazzi; Pierre Nicolas; Tom Wiklund; Jean-Francois Bernardet; Eric Duchaud

doi:10.3389/fmicb.2017.01752

Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme

Tatiana Rochat, Erina Fujiwara-Nagata, Ségolène Calvez, Inger Dalsgaard, Lone Madsen, Alexandra Calteau, Aurélie Lunazzi, Pierre Nicolas, Tom Wiklund, Jean-Francois Bernardet, Eric Duchaud

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Abstract

Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.

Original language	English
Article number	1752
Journal	Frontiers in Microbiology
Volume	8
ISSN	1664-302X
DOIs	https://doi.org/10.3389/fmicb.2017.01752
Publication status	Published - 2017

Bibliographical note

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

Keywords

mPCR
Fish-pathogenic bacteria
Flavobacterium psychrophilum
Genomics
Salmonid aquaculture
Serotype

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Rochat, T., Fujiwara-Nagata, E., Calvez, S., Dalsgaard, I., Madsen, L., Calteau, A., Lunazzi, A., Nicolas, P., Wiklund, T., Bernardet, J.-F., & Duchaud, E. (2017). Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme. Frontiers in Microbiology, 8, Article 1752. https://doi.org/10.3389/fmicb.2017.01752

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abstract = "Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.",

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year = "2017",

doi = "10.3389/fmicb.2017.01752",

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T1 - Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme

AU - Rochat, Tatiana

AU - Fujiwara-Nagata, Erina

AU - Calvez, Ségolène

AU - Dalsgaard, Inger

AU - Madsen, Lone

AU - Calteau, Alexandra

AU - Lunazzi, Aurélie

AU - Nicolas, Pierre

AU - Wiklund, Tom

AU - Bernardet, Jean-Francois

AU - Duchaud, Eric

N1 - This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

PY - 2017

Y1 - 2017

N2 - Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.

AB - Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.

KW - mPCR

KW - Fish-pathogenic bacteria

KW - Flavobacterium psychrophilum

KW - Genomics

KW - Salmonid aquaculture

KW - Serotype

U2 - 10.3389/fmicb.2017.01752

DO - 10.3389/fmicb.2017.01752

M3 - Journal article

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SN - 1664-302X

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 1752

ER -

Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme

Abstract

Bibliographical note

Keywords

UN SDGs

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