Genomic Characterization of Campylobacter jejuni strain M1

Carsten Friis; Gertrude Maria Wassenaar; Muhammad A. Javed; Lars Snipen; Karin Lagesen; Peter Fischer Hallin; Diane G. Newell; Monique Toszeghy; Anne Ridley; Georgia Manning; David Ussery

doi:10.1371/journal.pone.0012253

Genomic Characterization of Campylobacter jejuni strain M1

Carsten Friis, Gertrude Maria Wassenaar, Muhammad A. Javed, Lars Snipen, Karin Lagesen, Peter Fischer Hallin, Diane G. Newell, Monique Toszeghy, Anne Ridley, Georgia Manning, David Ussery

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Abstract

Campylobacter jejuni strain M1 (laboratory designation 99/308) is a rarely documented case of direct transmission of C. jejuni from chicken to a person, resulting in enteritis. We have sequenced the genome of C. jejuni strain M1, and compared this to 12 other C. jejuni sequenced genomes currently publicly available. Compared to these, M1 is closest to strain 81116. Based on the 13 genome sequences, we have identified the C. jejuni pan-genome, as well as the core genome, the auxiliary genes, and genes unique between strains M1 and 81116. The pan-genome contains 2,427 gene families, whilst the core genome comprised 1,295 gene families, or about two-thirds of the gene content of the average of the sequenced C. jejuni genomes. Various comparison and visualization tools were applied to the 13 C. jejuni genome sequences, including a species pan- and core genome plot, a BLAST Matrix and a BLAST Atlas. Trees based on 16S rRNA sequences and on the total gene families in each genome are presented. The findings are discussed in the background of the proven virulence potential of M1.

Original language	English
Journal	P L o S One
Volume	5
Issue number	8
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0012253
Publication status	Published - 2010

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Friis, C., Wassenaar, G. M., Javed, M. A., Snipen, L., Lagesen, K., Hallin, P. F., Newell, D. G., Toszeghy, M., Ridley, A., Manning, G., & Ussery, D. (2010). Genomic Characterization of Campylobacter jejuni strain M1. P L o S One, 5(8). https://doi.org/10.1371/journal.pone.0012253

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abstract = "Campylobacter jejuni strain M1 (laboratory designation 99/308) is a rarely documented case of direct transmission of C. jejuni from chicken to a person, resulting in enteritis. We have sequenced the genome of C. jejuni strain M1, and compared this to 12 other C. jejuni sequenced genomes currently publicly available. Compared to these, M1 is closest to strain 81116. Based on the 13 genome sequences, we have identified the C. jejuni pan-genome, as well as the core genome, the auxiliary genes, and genes unique between strains M1 and 81116. The pan-genome contains 2,427 gene families, whilst the core genome comprised 1,295 gene families, or about two-thirds of the gene content of the average of the sequenced C. jejuni genomes. Various comparison and visualization tools were applied to the 13 C. jejuni genome sequences, including a species pan- and core genome plot, a BLAST Matrix and a BLAST Atlas. Trees based on 16S rRNA sequences and on the total gene families in each genome are presented. The findings are discussed in the background of the proven virulence potential of M1.",

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Genomic Characterization of Campylobacter jejuni strain M1. / Friis, Carsten; Wassenaar, Gertrude Maria; Javed, Muhammad A.; Snipen, Lars; Lagesen, Karin; Hallin, Peter Fischer; Newell, Diane G.; Toszeghy, Monique; Ridley, Anne; Manning, Georgia; Ussery, David.

In: P L o S One, Vol. 5, No. 8, 2010.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Friis, Carsten

AU - Wassenaar, Gertrude Maria

AU - Javed, Muhammad A.

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AU - Lagesen, Karin

AU - Hallin, Peter Fischer

AU - Newell, Diane G.

AU - Toszeghy, Monique

AU - Ridley, Anne

AU - Manning, Georgia

AU - Ussery, David

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N2 - Campylobacter jejuni strain M1 (laboratory designation 99/308) is a rarely documented case of direct transmission of C. jejuni from chicken to a person, resulting in enteritis. We have sequenced the genome of C. jejuni strain M1, and compared this to 12 other C. jejuni sequenced genomes currently publicly available. Compared to these, M1 is closest to strain 81116. Based on the 13 genome sequences, we have identified the C. jejuni pan-genome, as well as the core genome, the auxiliary genes, and genes unique between strains M1 and 81116. The pan-genome contains 2,427 gene families, whilst the core genome comprised 1,295 gene families, or about two-thirds of the gene content of the average of the sequenced C. jejuni genomes. Various comparison and visualization tools were applied to the 13 C. jejuni genome sequences, including a species pan- and core genome plot, a BLAST Matrix and a BLAST Atlas. Trees based on 16S rRNA sequences and on the total gene families in each genome are presented. The findings are discussed in the background of the proven virulence potential of M1.

AB - Campylobacter jejuni strain M1 (laboratory designation 99/308) is a rarely documented case of direct transmission of C. jejuni from chicken to a person, resulting in enteritis. We have sequenced the genome of C. jejuni strain M1, and compared this to 12 other C. jejuni sequenced genomes currently publicly available. Compared to these, M1 is closest to strain 81116. Based on the 13 genome sequences, we have identified the C. jejuni pan-genome, as well as the core genome, the auxiliary genes, and genes unique between strains M1 and 81116. The pan-genome contains 2,427 gene families, whilst the core genome comprised 1,295 gene families, or about two-thirds of the gene content of the average of the sequenced C. jejuni genomes. Various comparison and visualization tools were applied to the 13 C. jejuni genome sequences, including a species pan- and core genome plot, a BLAST Matrix and a BLAST Atlas. Trees based on 16S rRNA sequences and on the total gene families in each genome are presented. The findings are discussed in the background of the proven virulence potential of M1.

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