TY - JOUR
T1 - Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance
AU - Munk, Patrick
AU - Brinch, Christian
AU - Møller, Frederik Duus
AU - Petersen, Thomas N.
AU - Hendriksen, Rene S.
AU - Seyfarth, Anne Mette
AU - Kjeldgaard, Jette S.
AU - Svendsen, Christina Aaby
AU - van Bunnik, Bram
AU - Berglund, Fanny
AU - Larsson, D. G. Joakim
AU - Koopmans, Marion
AU - Woolhouse, Mark
AU - Aarestrup, Frank M.
A2 - Global, Sewage Surveillance Consortium
PY - 2022
Y1 - 2022
N2 - Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic resistance genes (ARGs) is essential to develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing to analyse ARGs in 757 sewage samples from 243 cities in 101 countries, collected from 2016 to 2019. We find regional patterns in resistomes, and these differ between subsets corresponding to drug classes and are partly driven by taxonomic variation. The genetic environments of 49 common ARGs are highly diverse, with most common ARGs carried by multiple distinct genomic contexts globally and sometimes on plasmids. Analysis of flanking sequence revealed ARG-specific patterns of dispersal limitation and global transmission. Our data furthermore suggest certain geographies are more prone to transmission events and should receive additional attention.
AB - Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic resistance genes (ARGs) is essential to develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing to analyse ARGs in 757 sewage samples from 243 cities in 101 countries, collected from 2016 to 2019. We find regional patterns in resistomes, and these differ between subsets corresponding to drug classes and are partly driven by taxonomic variation. The genetic environments of 49 common ARGs are highly diverse, with most common ARGs carried by multiple distinct genomic contexts globally and sometimes on plasmids. Analysis of flanking sequence revealed ARG-specific patterns of dispersal limitation and global transmission. Our data furthermore suggest certain geographies are more prone to transmission events and should receive additional attention.
U2 - 10.1038/s41467-022-34312-7
DO - 10.1038/s41467-022-34312-7
M3 - Journal article
C2 - 36456547
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 7251
ER -