Abstract
| Original language | English |
|---|---|
| Article number | fny291 |
| Journal | F E M S Microbiology Letters |
| Volume | 366 |
| Issue number | 1 |
| Number of pages | 12 |
| ISSN | 0378-1097 |
| DOIs | |
| Publication status | Published - 2019 |
Bibliographical note
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedKeywords
- Genetic tool development
- Food fermentation
- Biotherapeutics
- Phytotherapeutics
- Synthetic biology
- GMO
Cite this
}
Genome editing of lactic acid bacteria: opportunities for food, feed, pharma and biotech. / Börner, Rosa A.; Kandasamy, Vijayalakshmi; Axelsen, Amalie M.; Nielsen, Alex T.; Bosma, Elleke F.
In: F E M S Microbiology Letters, Vol. 366, No. 1, fny291, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Genome editing of lactic acid bacteria: opportunities for food, feed, pharma and biotech
AU - Börner, Rosa A.
AU - Kandasamy, Vijayalakshmi
AU - Axelsen, Amalie M.
AU - Nielsen, Alex T.
AU - Bosma, Elleke F.
N1 - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
PY - 2019
Y1 - 2019
N2 - This mini-review provides a perspective of traditional, emerging, and future applications of lactic acid bacteria (LAB) and how genome editing tools can be used to overcome current challenges in all these applications. It also describes available tools and how these can be further developed, and takes current legislation into account. Genome editing tools are necessary for the construction of strains for new applications and products, but can also play a crucial role in traditional ones, such as food and probiotics, as a research tool for understanding mechanistic insights and discovering new properties. Traditionally, recombinant DNA techniques for LAB have strongly focused on being food-grade, but they lack speed and the number of genetically tractable strains is still rather limited. Further tool development will enable rapid construction of multiple mutants or mutant libraries on a genomic level in a wide variety of LAB strains. We also propose an iterative Design-Build-Test-Learn workflow cycle for LAB cell factory development based on systems biology, with “cell factory” expanding beyond its traditional meaning of production strains and making use of genome editing tools to advance LAB understanding, applications and strain development.
AB - This mini-review provides a perspective of traditional, emerging, and future applications of lactic acid bacteria (LAB) and how genome editing tools can be used to overcome current challenges in all these applications. It also describes available tools and how these can be further developed, and takes current legislation into account. Genome editing tools are necessary for the construction of strains for new applications and products, but can also play a crucial role in traditional ones, such as food and probiotics, as a research tool for understanding mechanistic insights and discovering new properties. Traditionally, recombinant DNA techniques for LAB have strongly focused on being food-grade, but they lack speed and the number of genetically tractable strains is still rather limited. Further tool development will enable rapid construction of multiple mutants or mutant libraries on a genomic level in a wide variety of LAB strains. We also propose an iterative Design-Build-Test-Learn workflow cycle for LAB cell factory development based on systems biology, with “cell factory” expanding beyond its traditional meaning of production strains and making use of genome editing tools to advance LAB understanding, applications and strain development.
KW - Genetic tool development
KW - Food fermentation
KW - Biotherapeutics
KW - Phytotherapeutics
KW - Synthetic biology
KW - GMO
U2 - 10.1093/femsle/fny291
DO - 10.1093/femsle/fny291
M3 - Journal article
VL - 366
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
SN - 0378-1097
IS - 1
M1 - fny291
ER -