@inbook{661386343cdb4412a550548b520c08bd,
title = "Genome editing: CRISPR-Cas9",
abstract = "In the present chapter, we present the protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place. Hence, we firstly explain how to identify dominant markers for genetic transformation. Secondly, we provide a guide for construction of Cas9/sgRNA episomal expression vectors. Thirdly, we present how to mutagenize reporter genes to explore the efficiency of CRISPR-Cas9 in the relevant fungus and to ease subsequent CRISPR-mediated genetic engineering. Lastly, we describe how to make CRISPR-mediated marker-dependent and marker-free gene targeting.",
keywords = "CRISPR, Gene editing, Gene targeting, Mutagenesis, Nonmodel fungi",
author = "Hoof, {Jakob B.} and N{\o}dvig, {Christina S.} and Mortensen, {Uffe H.}",
year = "2018",
doi = "10.1007/978-1-4939-7804-5_11",
language = "English",
isbn = "978-1-4939-7803-8",
volume = "1775",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "119--132",
editor = "{de Vries}, {Ronald P.} and Tsang, {Adrian } and Grigoriev, {Igor V.}",
booktitle = "Fungal Genomics: methods and protocols",
address = "United States",
edition = "2",
}