Genome analysis of Clostridium perfringens isolates from healthy and necrotic enteritis infected chickens and turkeys

Troels Ronco, Marc Stegger, Kim Lee Ng, Berit Lilje, Ulrike Lyhs, Paal Skytt Andersen, Karl Pedersen

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Abstract

Objective: Clostridium perfringens causes gastrointestinal diseases in both humans and domestic animals. Type A strains expressing the NetB toxin are the main cause of necrotic enteritis (NE) in chickens, which has remarkable impact on animal welfare and production economy in the international poultry industry. Three pathogenicity loci NELoc-1, -2 and -3 and a collagen adhesion gene cnaA have been found to be associated with NE in chickens, whereas the presence of these has not been investigated in diseased turkeys. The purpose was to investigate the virulence associated genome content and the genetic relationship among 30 C. perfringens isolates from both healthy and NE infected chickens and turkeys, applying whole-genome sequencing. Results: NELoc-1, -3, netB and cnaA were significantly associated with NE isolates from chickens, whereas only NELoc-2 was commonly observed in both diseased turkeys and chickens. A putative collagen adhesion gene that encodes a von Willebrand Factor (vWF) domain was identified in all diseased turkeys and designated as cnaD. The phylogenetic analysis based on single nucleotide polymorphisms showed that the isolates generally were not closely related. These results indicate that virulence factors and pathogenicity loci associated with NE in chickens are not important to the same extent in diseased turkeys except for NELoc-2. A putative collagen adhesion gene which potentially could be of importance in regard to the NE pathogenesis in turkeys was identified and need to be further investigated. Thus, the pathogenesis of NE in turkeys appears to be different from that of broiler chickens.
Original languageEnglish
Article number270
JournalBMC Research Notes
Volume10
ISSN1756-0500
DOIs
Publication statusPublished - 2017

Bibliographical note

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.

Keywords

  • Genome analysis
  • Necrotic enteritis
  • Poultry
  • Virulence factors

Cite this

Ronco, Troels ; Stegger, Marc ; Ng, Kim Lee ; Lilje, Berit ; Lyhs, Ulrike ; Andersen, Paal Skytt ; Pedersen, Karl. / Genome analysis of Clostridium perfringens isolates from healthy and necrotic enteritis infected chickens and turkeys. In: BMC Research Notes. 2017 ; Vol. 10.
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title = "Genome analysis of Clostridium perfringens isolates from healthy and necrotic enteritis infected chickens and turkeys",
abstract = "Objective: Clostridium perfringens causes gastrointestinal diseases in both humans and domestic animals. Type A strains expressing the NetB toxin are the main cause of necrotic enteritis (NE) in chickens, which has remarkable impact on animal welfare and production economy in the international poultry industry. Three pathogenicity loci NELoc-1, -2 and -3 and a collagen adhesion gene cnaA have been found to be associated with NE in chickens, whereas the presence of these has not been investigated in diseased turkeys. The purpose was to investigate the virulence associated genome content and the genetic relationship among 30 C. perfringens isolates from both healthy and NE infected chickens and turkeys, applying whole-genome sequencing. Results: NELoc-1, -3, netB and cnaA were significantly associated with NE isolates from chickens, whereas only NELoc-2 was commonly observed in both diseased turkeys and chickens. A putative collagen adhesion gene that encodes a von Willebrand Factor (vWF) domain was identified in all diseased turkeys and designated as cnaD. The phylogenetic analysis based on single nucleotide polymorphisms showed that the isolates generally were not closely related. These results indicate that virulence factors and pathogenicity loci associated with NE in chickens are not important to the same extent in diseased turkeys except for NELoc-2. A putative collagen adhesion gene which potentially could be of importance in regard to the NE pathogenesis in turkeys was identified and need to be further investigated. Thus, the pathogenesis of NE in turkeys appears to be different from that of broiler chickens.",
keywords = "Genome analysis, Necrotic enteritis, Poultry, Virulence factors",
author = "Troels Ronco and Marc Stegger and Ng, {Kim Lee} and Berit Lilje and Ulrike Lyhs and Andersen, {Paal Skytt} and Karl Pedersen",
note = "This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.",
year = "2017",
doi = "10.1186/s13104-017-2594-9",
language = "English",
volume = "10",
journal = "BMC Research Notes",
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Genome analysis of Clostridium perfringens isolates from healthy and necrotic enteritis infected chickens and turkeys. / Ronco, Troels; Stegger, Marc; Ng, Kim Lee; Lilje, Berit; Lyhs, Ulrike; Andersen, Paal Skytt; Pedersen, Karl.

In: BMC Research Notes, Vol. 10, 270, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Genome analysis of Clostridium perfringens isolates from healthy and necrotic enteritis infected chickens and turkeys

AU - Ronco, Troels

AU - Stegger, Marc

AU - Ng, Kim Lee

AU - Lilje, Berit

AU - Lyhs, Ulrike

AU - Andersen, Paal Skytt

AU - Pedersen, Karl

N1 - This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.

PY - 2017

Y1 - 2017

N2 - Objective: Clostridium perfringens causes gastrointestinal diseases in both humans and domestic animals. Type A strains expressing the NetB toxin are the main cause of necrotic enteritis (NE) in chickens, which has remarkable impact on animal welfare and production economy in the international poultry industry. Three pathogenicity loci NELoc-1, -2 and -3 and a collagen adhesion gene cnaA have been found to be associated with NE in chickens, whereas the presence of these has not been investigated in diseased turkeys. The purpose was to investigate the virulence associated genome content and the genetic relationship among 30 C. perfringens isolates from both healthy and NE infected chickens and turkeys, applying whole-genome sequencing. Results: NELoc-1, -3, netB and cnaA were significantly associated with NE isolates from chickens, whereas only NELoc-2 was commonly observed in both diseased turkeys and chickens. A putative collagen adhesion gene that encodes a von Willebrand Factor (vWF) domain was identified in all diseased turkeys and designated as cnaD. The phylogenetic analysis based on single nucleotide polymorphisms showed that the isolates generally were not closely related. These results indicate that virulence factors and pathogenicity loci associated with NE in chickens are not important to the same extent in diseased turkeys except for NELoc-2. A putative collagen adhesion gene which potentially could be of importance in regard to the NE pathogenesis in turkeys was identified and need to be further investigated. Thus, the pathogenesis of NE in turkeys appears to be different from that of broiler chickens.

AB - Objective: Clostridium perfringens causes gastrointestinal diseases in both humans and domestic animals. Type A strains expressing the NetB toxin are the main cause of necrotic enteritis (NE) in chickens, which has remarkable impact on animal welfare and production economy in the international poultry industry. Three pathogenicity loci NELoc-1, -2 and -3 and a collagen adhesion gene cnaA have been found to be associated with NE in chickens, whereas the presence of these has not been investigated in diseased turkeys. The purpose was to investigate the virulence associated genome content and the genetic relationship among 30 C. perfringens isolates from both healthy and NE infected chickens and turkeys, applying whole-genome sequencing. Results: NELoc-1, -3, netB and cnaA were significantly associated with NE isolates from chickens, whereas only NELoc-2 was commonly observed in both diseased turkeys and chickens. A putative collagen adhesion gene that encodes a von Willebrand Factor (vWF) domain was identified in all diseased turkeys and designated as cnaD. The phylogenetic analysis based on single nucleotide polymorphisms showed that the isolates generally were not closely related. These results indicate that virulence factors and pathogenicity loci associated with NE in chickens are not important to the same extent in diseased turkeys except for NELoc-2. A putative collagen adhesion gene which potentially could be of importance in regard to the NE pathogenesis in turkeys was identified and need to be further investigated. Thus, the pathogenesis of NE in turkeys appears to be different from that of broiler chickens.

KW - Genome analysis

KW - Necrotic enteritis

KW - Poultry

KW - Virulence factors

U2 - 10.1186/s13104-017-2594-9

DO - 10.1186/s13104-017-2594-9

M3 - Journal article

VL - 10

JO - BMC Research Notes

JF - BMC Research Notes

SN - 1756-0500

M1 - 270

ER -