Genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans

Ákos T. Kovács; Mariska Van Hartskamp; Oscar P. Kuipers; Richard Van Kranenburg

doi:10.1128/AEM.03060-09

Genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans

Ákos T. Kovács
, Mariska Van Hartskamp
, Oscar P. Kuipers
, Richard Van Kranenburg

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	12
Pages (from-to)	4085-4088
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.03060-09
Publication status	Published - 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

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10.1128/AEM.03060-09

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title = "Genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans",

abstract = "Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.",

author = "Kov{\'a}cs, \{{\'A}kos T.\} and \{Van Hartskamp\}, Mariska and Kuipers, \{Oscar P.\} and \{Van Kranenburg\}, Richard",

year = "2010",

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language = "English",

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publisher = "American Society for Microbiology",

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AU - Kovács, Ákos T.

AU - Van Hartskamp, Mariska

AU - Kuipers, Oscar P.

AU - Van Kranenburg, Richard

PY - 2010

Y1 - 2010

N2 - Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.

AB - Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.

U2 - 10.1128/AEM.03060-09

DO - 10.1128/AEM.03060-09

M3 - Journal article

C2 - 20400555

AN - SCOPUS:77953632718

SN - 0099-2240

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SP - 4085

EP - 4088

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 12

ER -

Genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans

Abstract

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