Genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans

Ákos T. Kovács, Mariska Van Hartskamp, Oscar P. Kuipers, Richard Van Kranenburg

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.

Original languageEnglish
JournalApplied and Environmental Microbiology
Volume76
Issue number12
Pages (from-to)4085-4088
ISSN0099-2240
DOIs
Publication statusPublished - 2010
Externally publishedYes

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