Genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans

Ákos T. Kovács; Mariska Van Hartskamp; Oscar P. Kuipers; Richard Van Kranenburg

doi:10.1128/AEM.03060-09

Genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans

Ákos T. Kovács, Mariska Van Hartskamp, Oscar P. Kuipers, Richard Van Kranenburg

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	12
Pages (from-to)	4085-4088
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.03060-09
Publication status	Published - 2010
Externally published	Yes

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abstract = "Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.",

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AU - Van Kranenburg, Richard

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AB - Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous D-lactate dehydrogenase expression.

U2 - 10.1128/AEM.03060-09

DO - 10.1128/AEM.03060-09

M3 - Journal article

C2 - 20400555

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JO - Applied and Environmental Microbiology

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SN - 0099-2240

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