Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes

Ilaria Benedetti, Victor de Lorenzo, Pablo Ivan Nikel

Research output: Contribution to journalJournal articleResearchpeer-review


Bacterial biofilms outperform planktonic counterparts in whole-cell biocatalysis. The transition between planktonic and biofilm lifestyles of the platform strain Pseudomonas putida KT2440 is ruled by a regulatory network controlling the levels of the trigger signal cyclic di-GMP (c-di-GMP). This circumstance was exploited for designing a genetic device that over-runs the synthesis or degradation of c-di-GMP thus making P. putida to form biofilms at user's will. For this purpose, the transcription of either yedQ (diguanylate cyclase) or yhjH (c-di-GMP phoshodiesterase) from Escherichia coli was artificially placed under the tight control of a cyclohexanone-responsive expression system. The resulting strain was subsequently endowed with a synthetic operon and tested for 1-chlorobutane biodegradation. Upon addition of cyclohexanone to the culture medium, the thereby designed P. putida cells formed biofilms displaying high dehalogenase activity. These results show that the morphologies and physical forms of whole-cell biocatalysts can be genetically programmed while purposely designing their biochemical activity. 
Original languageEnglish
JournalMetabolic Engineering
Pages (from-to)109-118
Number of pages10
Publication statusPublished - 2016
Externally publishedYes

Bibliographical note

© 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.


  • Biocatalysis
  • Catalytic biofilm
  • Dehalogenase
  • Metabolic engineering
  • Pseudomonas putida
  • Synthetic morphology


Dive into the research topics of 'Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes'. Together they form a unique fingerprint.

Cite this