Genetic heterogeneity of Campylobacter concisus determined by pulsed field gel electrophoresis-based macrorestriction profiling

M.I. Matsheka, B.G. Elisha, A.L. Lastovica, Stephen L.W. On

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In order to investigate the genetic diversity of Campylobacter concisus to assist molecular typing studies, the use of macrorestriction profiling was examined. A suitable protocol was developed that included the use of formaldehyde pretreatment to prevent DNA degradation, and restriction enzyme Not1 for pulsed field gel electrophoresis-based genotyping. Subsequently, 53 strains of C concisus, principally from cases of diarrhoea in children, were examined. Fifty-one distinct patterns were obtained, indicating the high discriminatory potential of the method. Patterns comprised between one and 14 restriction fragments, with type and reference strains of two well-defined genomospecies of oral and faecal origin containing six and 12 fragments respectively. Our results show that C concisus is genetically diverse and suggest the species as currently defined to be a taxonomic continuum comprised of several genomospecies. The pulsed field gel electrophoresis typing method described here has considerable potential for molecular epidemiological studies of C concisus and may be a useful adjunctive method for helping to resolve key taxonomic issues for this species.
Original languageEnglish
JournalFEMS Microbiology Letters
Volume211
Issue number1
Pages (from-to)17-22
ISSN0378-1097
DOIs
Publication statusPublished - 2002

Keywords

  • Campylobacter concisus
  • typing
  • genetic diversity
  • PFGE

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