TY - JOUR
T1 - Genetic engineering approaches to improve post-translational modification of biopharmaceuticals in different production platforms
AU - Amann, Thomas
AU - Schmieder, Valerie
AU - Kildegaard, Helene Faustrup
AU - Borth, Nicole
AU - Andersen, Mikael Rørdam
PY - 2019
Y1 - 2019
N2 - The number of approved biopharmaceuticals, where product quality attributes remain of major importance, is increasing steadily. Within the available variety of expression hosts, the production of biopharmaceuticals faces diverse limitations with respect to post-translational modifications (PTM), while different biopharmaceuticals demand different forms and specifications of PTMs for proper functionality. With the growing toolbox of genetic engineering technologies, it is now possible to address general as well as host- or biopharmaceutical-specific product quality obstacles. In this review we present diverse expression systems derived from mammalians, bacteria, yeast, plants, and insects as well as available genetic engineering tools. We focus on genes for knockout / knockdown and overexpression for meaningful approaches to improve biopharmaceutical PTMs and discuss their applicability as well as future trends in the field. This article is protected by copyright. All rights reserved.
AB - The number of approved biopharmaceuticals, where product quality attributes remain of major importance, is increasing steadily. Within the available variety of expression hosts, the production of biopharmaceuticals faces diverse limitations with respect to post-translational modifications (PTM), while different biopharmaceuticals demand different forms and specifications of PTMs for proper functionality. With the growing toolbox of genetic engineering technologies, it is now possible to address general as well as host- or biopharmaceutical-specific product quality obstacles. In this review we present diverse expression systems derived from mammalians, bacteria, yeast, plants, and insects as well as available genetic engineering tools. We focus on genes for knockout / knockdown and overexpression for meaningful approaches to improve biopharmaceutical PTMs and discuss their applicability as well as future trends in the field. This article is protected by copyright. All rights reserved.
KW - CRISPR
KW - Cell Engineering
KW - Chinese Hamster Ovary
KW - Expression System
KW - Post-translational Modification
U2 - 10.1002/bit.27101
DO - 10.1002/bit.27101
M3 - Journal article
C2 - 31237682
SN - 0006-3592
VL - 116
SP - 2778
EP - 2796
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 10
ER -