Genetic Characterization of porcine circovirus type 2 isolated from different pig-farms in Croatia

Nevenka Rudan, Charlotte Kristiane Hjulsager, Kitt Dupont, Branko Bacanek, Evica Markovic, Josip Madic

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Histopathological fifi ndings in 25 pig tissue samples, which indicated PCVD (porcine circovirus diseases), were studied. Pig tissue samples originated from 5 different pig-farms in the north-west part of Croatia. Histopathological lesions showed two clinical pictures of the disease: porcine multisystemic wasting syndrome (PMWS) and porcine dermatitis nephropathy syndrome (PDNS). All samples were tested by PCR for the presence of porcine circovirus type 2 (PCV2). Twenty of them were PCV2 positive. PCV2 DNA was quantififi ed by realtime-PCR in all twenty samples with a wide range of PCV2 loads ranging from 105 to 1010 PCV2 genomes/μL. The highest PCV2 loads were detected in pigs with PMWS lesions, while PDNS affected pigs had lower PCV2 loads. The 20 PCV2 isolates were sequenced and analyzed. Nineteen isolates belonged to PCV2 group 1 and only one to PCV2 group 2. We found no link between genotypes
    Original languageEnglish
    JournalVeterinarski Arhiv
    Volume79
    Issue number4
    Pages (from-to)361 - 369
    ISSN0372-5480
    Publication statusPublished - 2009

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