Genetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation

Yudai Matsuda; Charlotte Held Gotfredsen; Thomas Ostenfeld Larsen

doi:10.1021/acs.orglett.8b03123

Genetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation

Yudai Matsuda, Charlotte Held Gotfredsen, Thomas Ostenfeld Larsen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor. Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.

Original language	English
Journal	ORGANIC LETTERS
Volume	20
Issue number	22
Pages (from-to)	7194-7200
Number of pages	4
ISSN	1523-7060
DOIs	https://doi.org/10.1021/acs.orglett.8b03123
Publication status	Published - 2018

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title = "Genetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation",

abstract = "A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor. Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.",

author = "Yudai Matsuda and Gotfredsen, {Charlotte Held} and Larsen, {Thomas Ostenfeld}",

year = "2018",

doi = "10.1021/acs.orglett.8b03123",

language = "English",

volume = "20",

pages = "7194--7200",

journal = "Organic Letters",

issn = "1523-7060",

publisher = "American Chemical Society",

number = "22",

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T1 - Genetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation

AU - Matsuda, Yudai

AU - Gotfredsen, Charlotte Held

AU - Larsen, Thomas Ostenfeld

PY - 2018

Y1 - 2018

N2 - A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor. Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.

AB - A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor. Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.

U2 - 10.1021/acs.orglett.8b03123

DO - 10.1021/acs.orglett.8b03123

M3 - Journal article

C2 - 30394754

VL - 20

SP - 7194

EP - 7200

JO - Organic Letters

JF - Organic Letters

SN - 1523-7060

IS - 22

ER -