Genes Linked to Production of Secondary Metabolites in Talaromyces atroroseus Revealed Using CRISPR-Cas9

Maria Lund Nielsen, Thomas Isbrandt Petersen, Kasper Bøwig Rasmussen, Ulf Thrane, Jakob Blæsbjerg Hoof, Thomas Ostenfeld Larsen, Uffe Hasbro Mortensen

Research output: Contribution to journalJournal articleResearchpeer-review

323 Downloads (Pure)

Abstract

The full potential of fungal secondary metabolism has until recently been impeded by the lack of universal genetic tools for most species. However, the emergence of several CRISPR-Cas9-based genome editing systems adapted for several genera of filamentous fungi have now opened the doors for future efforts in discovery of novel natural products and elucidation and engineering of their biosynthetic pathways in fungi where no genetic tools are in place. So far, most studies have focused on demonstrating the performance of CRISPR-Cas9 in various fungal model species, and recently we presented a versatile CRISPR-Cas9 system that can be successfully applied in several diverse Aspergillus species. Here we take it one step further and show that our system can be used also in a phylogenetically
distinct and largely unexplored species from the genus of Talaromyces. Specifically, we exploit CRISPR-Cas9-based genome editing to identify a new gene in T. atroroseus responsible for production of polyketide-nonribosomal peptide hybrid products, hence, linking fungal secondary metabolites to their genetic origin in a species where no genetic engineering has previously been performed.
Original languageEnglish
Article numbere0169712
JournalP L o S One
Volume12
Issue number1
Number of pages9
ISSN1932-6203
DOIs
Publication statusPublished - 2017

Cite this

@article{e3681227152247318bbabb525c8ff00b,
title = "Genes Linked to Production of Secondary Metabolites in Talaromyces atroroseus Revealed Using CRISPR-Cas9",
abstract = "The full potential of fungal secondary metabolism has until recently been impeded by the lack of universal genetic tools for most species. However, the emergence of several CRISPR-Cas9-based genome editing systems adapted for several genera of filamentous fungi have now opened the doors for future efforts in discovery of novel natural products and elucidation and engineering of their biosynthetic pathways in fungi where no genetic tools are in place. So far, most studies have focused on demonstrating the performance of CRISPR-Cas9 in various fungal model species, and recently we presented a versatile CRISPR-Cas9 system that can be successfully applied in several diverse Aspergillus species. Here we take it one step further and show that our system can be used also in a phylogeneticallydistinct and largely unexplored species from the genus of Talaromyces. Specifically, we exploit CRISPR-Cas9-based genome editing to identify a new gene in T. atroroseus responsible for production of polyketide-nonribosomal peptide hybrid products, hence, linking fungal secondary metabolites to their genetic origin in a species where no genetic engineering has previously been performed.",
author = "Nielsen, {Maria Lund} and Petersen, {Thomas Isbrandt} and Rasmussen, {Kasper B{\o}wig} and Ulf Thrane and Hoof, {Jakob Bl{\ae}sbjerg} and Larsen, {Thomas Ostenfeld} and Mortensen, {Uffe Hasbro}",
year = "2017",
doi = "10.1371/journal.pone.0169712",
language = "English",
volume = "12",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

Genes Linked to Production of Secondary Metabolites in Talaromyces atroroseus Revealed Using CRISPR-Cas9. / Nielsen, Maria Lund; Petersen, Thomas Isbrandt; Rasmussen, Kasper Bøwig; Thrane, Ulf; Hoof, Jakob Blæsbjerg; Larsen, Thomas Ostenfeld; Mortensen, Uffe Hasbro.

In: P L o S One, Vol. 12, No. 1, e0169712, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Genes Linked to Production of Secondary Metabolites in Talaromyces atroroseus Revealed Using CRISPR-Cas9

AU - Nielsen, Maria Lund

AU - Petersen, Thomas Isbrandt

AU - Rasmussen, Kasper Bøwig

AU - Thrane, Ulf

AU - Hoof, Jakob Blæsbjerg

AU - Larsen, Thomas Ostenfeld

AU - Mortensen, Uffe Hasbro

PY - 2017

Y1 - 2017

N2 - The full potential of fungal secondary metabolism has until recently been impeded by the lack of universal genetic tools for most species. However, the emergence of several CRISPR-Cas9-based genome editing systems adapted for several genera of filamentous fungi have now opened the doors for future efforts in discovery of novel natural products and elucidation and engineering of their biosynthetic pathways in fungi where no genetic tools are in place. So far, most studies have focused on demonstrating the performance of CRISPR-Cas9 in various fungal model species, and recently we presented a versatile CRISPR-Cas9 system that can be successfully applied in several diverse Aspergillus species. Here we take it one step further and show that our system can be used also in a phylogeneticallydistinct and largely unexplored species from the genus of Talaromyces. Specifically, we exploit CRISPR-Cas9-based genome editing to identify a new gene in T. atroroseus responsible for production of polyketide-nonribosomal peptide hybrid products, hence, linking fungal secondary metabolites to their genetic origin in a species where no genetic engineering has previously been performed.

AB - The full potential of fungal secondary metabolism has until recently been impeded by the lack of universal genetic tools for most species. However, the emergence of several CRISPR-Cas9-based genome editing systems adapted for several genera of filamentous fungi have now opened the doors for future efforts in discovery of novel natural products and elucidation and engineering of their biosynthetic pathways in fungi where no genetic tools are in place. So far, most studies have focused on demonstrating the performance of CRISPR-Cas9 in various fungal model species, and recently we presented a versatile CRISPR-Cas9 system that can be successfully applied in several diverse Aspergillus species. Here we take it one step further and show that our system can be used also in a phylogeneticallydistinct and largely unexplored species from the genus of Talaromyces. Specifically, we exploit CRISPR-Cas9-based genome editing to identify a new gene in T. atroroseus responsible for production of polyketide-nonribosomal peptide hybrid products, hence, linking fungal secondary metabolites to their genetic origin in a species where no genetic engineering has previously been performed.

U2 - 10.1371/journal.pone.0169712

DO - 10.1371/journal.pone.0169712

M3 - Journal article

C2 - 28056079

VL - 12

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 1

M1 - e0169712

ER -