Abstract
A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin,
defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected
regions was obtained by material-specific adsorption of thiol-modified polyethyleneglycol
thiol-PEG on gold followed by adsorption of poly-L-lysine PLL modified PEG PLL-g-PEG on
SiO2. Selective streptavidin binding to either gold or SiO2 or both was ensured by introducing
biotin-modified thiolated thiol-biotin and/or biotin-modified PLL-g-PEG PLL-g-PEGbiotin
compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the
macroscopic scale, the protein-adsorption-resistant properties and the streptavidin-binding capacity
were optimized using quartz crystal microbalance with dissipation monitoring. The reproduction of
micrometer-scale gold patterns on SiO2 into patterns of streptavidin was verified using fluorescence
microscopy, while the compatibility of the material-specific surface-modification strategy with
nanoscale features was accomplished by modifying a localized surface plasmon resonance LSPR
active template, defined by randomly distributed nanoapertures in a thin gold film on SiO2. The
demonstrated compatibility of the latter substrate with LSPR-based label-free sensing of
biorecognition reactions, combined with the fact that all compounds utilized are commercially
available, makes the surface-modification protocol attractive as a generic surface modification
solution for a broad range of biorecognition-based assays.
Original language | English |
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Journal | Biointerphases |
Volume | 2 |
Issue number | 1 |
Pages (from-to) | 49-55 |
ISSN | 1934-8630 |
DOIs | |
Publication status | Published - 2007 |
Externally published | Yes |