Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska; Sofie Wendel; Emil Christian Fischer; Morten H. H. Nørholm; Antoine Danchin

doi:10.1038/s41598-016-0005-4

Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska
, Sofie Wendel
, Emil Christian Fischer
, Morten H. H. Nørholm
, Antoine Danchin

CHU, Paris

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.

Original language	English
Journal	Scientific Reports
Volume	6
Issue number	1
Number of pages	7
ISSN	2045-2322
DOIs	https://doi.org/10.1038/s41598-016-0005-4
Publication status	Published - 2016

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BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production
Molin, S. (Project Manager), Nørholm, M. (Project Manager), Cavaleiro, M. (PhD Student), Machado, H. (PhD Student), Wendel, S. (PhD Student), van der Helm, E. (PhD Student), Bojanovic, K. (PhD Student), Vazquez Albacete, D. (Project Participant), D'Arrigo, I. (PhD Student), Li, S. (PhD Student), Yang, X. (PhD Student), Redl, S. M. A. (PhD Student), Pogrebnyakov, I. (PhD Student), Lehning, C. E. (PhD Student), Giubergia, S. (PhD Student), Calero Valdayo, P. M. (PhD Student) & Lohmann, R. (Project Manager)
01/10/2012 → 31/01/2017
Project: Research

Cite this

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title = "Generation of mutation hotspots in ageing bacterial colonies",

abstract = "How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.",

author = "Agnieszka Sekowska and Sofie Wendel and \{Christian Fischer\}, Emil and N{\o}rholm, \{Morten H. H.\} and Antoine Danchin",

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AU - Sekowska, Agnieszka

AU - Wendel, Sofie

AU - Christian Fischer, Emil

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AU - Danchin, Antoine

PY - 2016

Y1 - 2016

N2 - How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.

AB - How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.

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Generation of mutation hotspots in ageing bacterial colonies

Abstract

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BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production

Cite this